ArticlePloS one2014
Fra2 is a co-regulator of Fep1 inhibition in response to iron starvation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- The Hemoprotein Hhy1 Promotes Heme-Dependent Catalase Activity of Ctt1.Molecular microbiology · 2026Article
- Monothiol glutaredoxins in microbial iron metabolism: From iron-sulfur cluster trafficking to iron-dependent regulation.Advances in microbial physiology · 2026Review
- Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth.Frontiers in microbiology · 2025Article
- TheFrontiers in microbiology · 2024Article
- Sib1, Sib2, and Sib3 proteins are required for ferrichrome-mediated cross-feeding interaction betweenFrontiers in microbiology · 2022Article
- Non-mitochondrial aconitase regulates the expression of iron-uptake genes by controlling the RNA turnover process in fission yeast.Journal of microbiology (Seoul, Korea) · 2021Article
- Iron-sulfur cluster biogenesis, trafficking, and signaling: Roles for CGFS glutaredoxins and BolA proteins.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Management versus miscues in the cytosolic labile iron pool: The varied functions of iron chaperones.Biochimica et biophysica acta. Molecular cell research · 2020Review
- Sinorhizobium meliloti YrbA binds divalent metal cations using two conserved histidines.Bioscience reports · 2020Article
- Proteomic profiling of the monothiol glutaredoxin Grx3 reveals its global role in the regulation of iron dependent processes.PLoS genetics · 2020Article
- Iron-sulfur cluster signaling: The common thread in fungal iron regulation.Current opinion in chemical biology · 2020Review
- The monothiol glutaredoxin GrxD is essential for sensing iron starvation in Aspergillus fumigatus.PLoS genetics · 2019Article
- Is There a Role for Glutaredoxins and BOLAs in the Perception of the Cellular Iron Status in Plants?Frontiers in plant science · 2019Article
- Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human PathogenFrontiers in microbiology · 2018Article
- Comparison of structures amongGenes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Review
- Schizosaccharomyces pombe Grx4 regulates the transcriptional repressor Php4 via [2Fe-2S] cluster binding.Metallomics : integrated biometal science · 2017Article
- Mechanisms of iron sensing and regulation in the yeast Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2017Review
- A Glutaredoxin·BolA Complex Serves as an Iron-Sulfur Cluster Chaperone for the Cytosolic Cluster Assembly Machinery.The Journal of biological chemistry · 2016Article
- Cuf2 Is a Transcriptional Co-Regulator that Interacts with Mei4 for Timely Expression of Middle-Phase Meiotic Genes.PloS one · 2016Article
- Cellular sensing and transport of metal ions: implications in micronutrient homeostasis.The Journal of nutritional biochemistry · 2015Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron is required for several metabolic functions involved in cellular growth. Although several players involved in iron transport have been identified, the mechanisms by which iron-responsive transcription factors are controlled are still poorly understood. In Schizosaccharomyces pombe, the Fep1 transcription factor represses genes involved in iron acquisition in response to high levels of iron. In contrast, when iron levels are low, Fep1 becomes inactive and loses its ability to associate with chromatin. Although the molecular basis by which Fep1 is inactivated under iron starvation remains unknown, this process requires the monothiol glutaredoxin Grx4. Here, we demonstrate that Fra2 plays a role in the negative regulation of Fep1 activity. Disruption of fra2+ (fra2Δ) led to a constitutive repression of the fio1+ gene transcription. Fep1 was consistently active and constitutively bound to its target gene promoters in cells lacking fra2+. A constitutive activation of Fep1 was also observed in a php4Δ fra2Δ double mutant strain in which the behavior of Fep1 is freed of its transcriptional regulation by Php4. Microscopic analyses of cells expressing a functional Fra2-Myc13 protein revealed that Fra2 localized throughout the cells with a significant proportion of Fra2 being observed within the nuclei. Further analysis by coimmunoprecipitation showed that Fra2, Fep1 and Grx4 are associated in a heteroprotein complex. Bimolecular fluorescence complementation experiments brought further evidence that an interaction between Fep1 and Fra2 occurs in the nucleus. Taken together, results reported here revealed that Fra2 plays a role in the Grx4-mediated pathway that inactivates Fep1 in response to iron deficiency.
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