Evidence map›Paper›PMID 24896819›Full record

ArticleThe New England journal of medicine2014

Actionable diagnosis of neuroleptospirosis by next-generation sequencing.

Michael R Wilson, Samia N Naccache, Erik Samayoa, Mark Biagtan, Hiba Bashir, Guixia Yu, Shahriar M Salamat, Sneha Somasekar, Scot Federman, Steve Miller and 12 more

4 registry-linked trialsOpen access · bronzeAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in The New England journal of medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 4 registered trials, which are not on this map. Cited by 537 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
537citing papers in PubMed, 6 pooled it
46.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02910037 nacompletednot on this mapstarted 2016, after this paper: background citation

Clinical Implementation of Metagenomic Next-Generation Sequencing for Precision Diagnosis of Acute Infectious Diseases; Neuroinflammatory Cohort

TypeinterventionalSponsorUniversity of California, San FranciscoRan2016 to 2017Enrolled214ConditionsEncephalitis, MeningitisArmsmNGS for pathogen detection
NCT05581160 nacompletednot on this mapstarted 2022, after this paper: background citation

Assess the Performance of Metagenomic Sequencing in the Diagnosis of STI (NGS-IST)

TypeinterventionalSponsorInstitut de Médecine et d'Epidémiologie Appliquée - Fondation Internationale Léon M'BaRan2022 to 2024Enrolled332ConditionsSexually Transmitted Infections (Not HIV or Hepatitis), Chlamydia Trachomatis Infection, Neisseria Gonorrhoeae Infection, Mycoplasma Genitalium InfectionArmsAssess the Performance of Metagenomic Sequencing in the Diagnosis of STI (NGS-IST)
NCT07435649 narecruitingnot on this mapstarted 2026, after this paper: background citation

Investigation of the Delve Detect Cerebrospinal Fluid (CSF) Metagenomic Next-generation Sequencing (mNGS) Test When Used for the Initial Diagnostic Workup of Patients With Suspected Central Nervous System (CNS) Infection

TypeinterventionalSponsorDelve Bio, Inc.Ran2026 to 2027Enrolled400ConditionsCentral Nervous System InfectionArmsDelve Detect CSF mNGS Test, Real-World Data
NCT07626073 enrolling by invitationnot on this mapstarted 2023, after this paper: background citation

h Can Provide the Detection Results of Pathogen Type and Resistant Gene. This Study is a Prospective Multi Centers Study to Explore Using the New Diagnostic Tool of " RPIP " and the Different Detection Methods of Next Generation Sequencing and Multiplex PC R in Patients With Pneumonia in ICU. Further Compare the Difference of Pathogen Identification and Clinical Impact by Different Diagnostic Methods.

Typeobservational_patient_registrySponsorChina Medical University HospitalRan2023 to 2028Enrolled200ConditionsRespiratory Pathogens
3 · Its place in the literature

Who cites it

537 citing papers in PubMed, 6 syntheses or guidelines pooled it, 1,014 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Pooled it
  5. Trends and advances inFrontiers in microbiology · 2024
    Pooled it
  6. Metagenomic next-generation sequencing forFrontiers in public health · 2023
    Pooled it
  7. Trial
  8. First case of neonatal bloodstream infection caused byExperimental and therapeutic medicine · 2026
    Article
  9. Review
  10. Article
  11. Article
  12. Observational
  13. Article
  14. Article
  15. Review
  16. Microorganisms · 2026
    Review
  17. Article
  18. Article
  19. Article
  20. Article

477 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 6 institutions in 2 countries.

Michael R WilsonFrom the Departments of Biochemistry and Biophysics (M.R.W., J.L.D.), Neurology (M.R.W.), and Laboratory Medicine (S.N.N., E.S., G.Y., S.S., S.F., S.M., C.Y.C.), and the Department of Medicine, Division of Infectious Diseases (C.Y.C.), University of California, San Francisco (UCSF), and UCSF-Abbott Viral Diagnostics and Discovery Center (S.N.N., E.S., G.Y., S.S., S.F., S.M., C.Y.C.) - both in San Francisco; the Department of Medicine, Division of Allergy and Immunology (M.B., H.B., J.E.G.), and the Departments of Pathology and Laboratory Medicine (S.M.S., K.D.R.) and Pediatrics (T.L.M., C.M.S., S.L.H., J.E.G.), University of Wisconsin, Madison; the Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD (R.S., E.G., F.C.); the Departments of Pediatrics and Immunology, Division of Allergy and Immunology, Duke University, Durham, NC (R.H.B.); and the Centers for Disease Control and Prevention, Atlanta (R.G.).
Samia N Naccache
Erik Samayoa
Mark Biagtan
Hiba Bashir
Guixia Yu
Shahriar M Salamat
Sneha Somasekar
Scot Federman
Steve Miller
Robert Sokolic
Elizabeth Garabedian
Fabio Candotti
Rebecca H Buckley
Kurt D Reed
Teresa L Meyer
Christine M Seroogy
Renee Galloway
Sheryl L Henderson
James E Gern
Joseph L DeRisi
Charles Y Chiu
Abbott (Germany) · DEPediatrics and Genetics · USNational Cancer Institute · USCenter for Cancer Research · USDuke University · USUnited States Nuclear Regulatory Commission · US

Funding

Molecular and Clinical Studies of Primary ImmunodeficienciesZIAHG000121 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI CANDOTTI, FABIO · 2009 to 2014
$6.2M
Pan-Genomic Approaches for Comprehensive Blood Screening of Novel or Emerging Infectious AgentsR01HL105704 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHIU, CHARLES YEN · 2011 to 2020
$5.9M
Howard Hughes Medical InstituteIntramural NIH HHSNHLBI NIH HHS R01 HL105704NHLBI NIH HHS R01-HL105704
6 · The paper itself

Abstract

A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were negative. Targeted antimicrobial agents were administered, and the patient was discharged home 32 days later with a status close to his premorbid condition. Polymerase-chain-reaction (PCR) and serologic testing at the Centers for Disease Control and Prevention (CDC) subsequently confirmed evidence of Leptospira santarosai infection.

Indexed as

Adenosine DeaminaseAdolescentAgammaglobulinemiaBiopsyBrainCerebrospinal FluidDNA, BacterialFeverHeadacheHumansLeptospiraLeptospirosisMaleMeningoencephalitisSequence Analysis, DNASevere Combined ImmunodeficiencyAdenosine DeaminaseDNA, Bacterial

Identifiers

PMID24896819
PMCPMC4134948
OpenAlexW2106780559

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.