ArticleTheScientificWorldJournal2014
Ultrastructure of plant leaf cuticles in relation to sample preparation as observed by transmission electron microscopy.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Toward the crystallographic and microstructural mechanisms of plant leaf waxes as diffusion barriers.CrystEngComm · 2026Review
- Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.Journal of experimental botany · 2024Article
- Article
- Combined morphological and multi-omics analyses to reveal the developmental mechanism ofFrontiers in plant science · 2022Article
- Developmental Plasticity of the Amphibious LiverwortFrontiers in plant science · 2022Article
- Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles.Plant methods · 2021Article
- Water Sorption and Desorption of Isolated Cuticles From Three Woody Species With Focus onFrontiers in plant science · 2021Article
- A Guide to Elucidate the Hidden Multicomponent Layered Structure of Plant Cuticles by Raman Imaging.Frontiers in plant science · 2021Article
- Effects of ozone on cell organelles of alfalfa (Saudi journal of biological sciences · 2020Article
- A simple technique for assessing the cuticular diffusion of humic acid biostimulants.Plant methods · 2019Article
- Non-Invasive Examination of Plant Surfaces by Opto-Electronic Means--Using Russet as a Prime Example.Sensors (Basel, Switzerland) · 2016Article
- Cuticle Structure in Relation to Chemical Composition: Re-assessing the Prevailing Model.Frontiers in plant science · 2016Review
- Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition.Protoplasma · 2015Article
- Wettability, polarity, and water absorption of holm oak leaves: effect of leaf side and age.Plant physiology · 2014Article
- Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region.Frontiers in plant science · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr's resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.