ArticleLab on a chip2014
Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes.
Article in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 133 citations in OpenAlex.
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- Isolation Techniques of Micro/Nano-Scaled Species for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Robust and efficient separation of white blood cells from blood using a microfluidic chip with a pair of linearly tapered crossflow filter arrays.Mikrochimica acta · 2024Article
- Article
- Recent advances in microfluidic cell separation to enable centrifugation-free, low extracorporeal volume leukapheresis in pediatric patients.Blood transfusion = Trasfusione del sangue · 2023Review
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Liposomes or Extracellular Vesicles: A Comprehensive Comparison of Both Lipid Bilayer Vesicles for Pulmonary Drug Delivery.Polymers · 2023Review
- Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel.Cyborg and bionic systems (Washington, D.C.) · 2023Article
- Biomedical Applications of Microfluidic Devices: A Review.Biosensors · 2022Review
- A high-throughput microfluidic device based on controlled incremental filtration to enable centrifugation-free, low extracorporeal volume leukapheresis.Scientific reports · 2022Article
- Nanoporous membrane fabrication by nanoimprint lithography for nanoparticle sieving.Nanoscale advances · 2022Article
- [Research progress in the application of external field separation technology and microfluidic technology in the separation of micro/nanoscales].Se pu = Chinese journal of chromatography · 2021Review
- A Novel Microfluidic Device for Blood Plasma Filtration.Micromachines · 2021Article
- Cyclic tangential flow filtration system for isolation of extracellular vesicles.APL bioengineering · 2021Article
- Microfluidic Viscometer Using a Suspending Micromembrane for Measurement of Biosamples.Micromachines · 2020Article
- Immunomagnetic separation of circulating tumor cells with microfluidic chips and their clinical applications.Biomicrofluidics · 2020Review
- Disease diagnostics using hydrodynamic flow focusing in microfluidic devices: Beyond flow cytometry.Biomedical engineering letters · 2020Review
- High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces.Micromachines · 2020Article
- Flow induced particle separation and collection through linear array pillar microfluidics device.Biomicrofluidics · 2020Article
- Label-free microfluidic sorting of microparticles.APL bioengineering · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
White blood cells (WBCs) constitute about 0.1% of the blood cells, yet they play a critical role in innate and adaptive immune responses against pathogenic infections, allergic conditions, and malignancies and thus contain rich information about the immune status of the body. Rapid isolation of WBCs directly from whole blood is a prerequisite for any integrated immunoassay platform designed for examining WBC phenotypes and functions; however, such functionality is still challenging for blood-on-a-chip systems, as existing microfluidic cell sorting techniques are inadequate for efficiently processing unprocessed whole blood on chip with concurrent high throughput and cell purity. Herein we report a microfluidic chip for continuous-flow isolation and sorting of WBCs from whole blood with high throughput and separation efficiency. The microfluidic cell sorting chip leveraged the crossflow filtration scheme in conjunction with a surface-micromachined poly(dimethylsiloxane) (PDMS) microfiltration membrane (PMM) with high porosity. With a sample throughput of 1 mL h(-1), the microfluidic cell sorting chip could recover 27.4 ± 4.9% WBCs with a purity of 93.5 ± 0.5%. By virtue of its separation efficiency, ease of sample recovery, and high throughput enabled by its continuous-flow operation, the microfluidic cell sorting chip holds promise as an upstream component for blood sample preparation and analysis in integrated blood-on-a-chip systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.