ArticleMolecular microbiology2014
A chemical potentiator of copper-accumulation used to investigate the iron-regulons of Saccharomyces cerevisiae.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Role of horizontally transferred copper resistance genes inMicrobiology (Reading, England) · 2022Review
- Getting out what you put in: Copper in mitochondria and its impacts on human disease.Biochimica et biophysica acta. Molecular cell research · 2021Review
- A copper(II)-binding triazole derivative with ionophore properties is active against Candida spp.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2020Article
- Increasing the copper sensitivity of microorganisms by restricting iron supply, a strategy for bio-management practices.Microbial biotechnology · 2020Article
- Reconstitution of a thermophilic CuThe Journal of biological chemistry · 2018Article
- Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast.PLoS biology · 2018Article
- Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess.Cell chemical biology · 2017Article
- Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae.Open biology · 2016Article
- The late-annotated small ORF LSO1 is a target gene of the iron regulon of Saccharomyces cerevisiae.MicrobiologyOpen · 2015Article
- Mitochondrial Iron-Sulfur Cluster Activity and Cytosolic Iron Regulate Iron Traffic in Saccharomyces cerevisiae.The Journal of biological chemistry · 2015Article
- Copper at the Fungal Pathogen-Host Axis.The Journal of biological chemistry · 2015Review
- A genome-wide copper-sensitized screen identifies novel regulators of mitochondrial cytochrome c oxidase activity.The Journal of biological chemistryArticle
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
The extreme resistance of Saccharomyces cerevisiae to copper is overcome by 2-(6-benzyl-2-pyridyl)quinazoline (BPQ), providing a chemical-biology tool which has been exploited in two lines of discovery. First, BPQ is shown to form a red (BPQ)2 Cu(I) complex and promote Ctr1-independent copper-accumulation in whole cells and in mitochondria isolated from treated cells. Multiple phenotypes, including loss of aconitase activity, are consistent with copper-BPQ mediated damage to mitochondrial iron-sulphur clusters. Thus, a biochemical basis of copper-toxicity in S. cerevisiae is analogous to other organisms. Second, iron regulons controlled by Aft1/2, Cth2 and Yap5 that respond to mitochondrial iron-sulphur cluster status are modulated by copper-BPQ causing iron hyper-accumulation via upregulated iron-import. Comparison of copper-BPQ treated, untreated and copper-only treated wild-type and fra2Δ by RNA-seq has uncovered a new candidate Aft1 target-gene (LSO1) and paralogous non-target (LSO2), plus nine putative Cth2 target-transcripts. Two lines of evidence confirm that Fra2 dominates basal repression of the Aft1/2 regulons in iron-replete cultures. Fra2-independent control of these regulons is also observed but CTH2 itself appears to be atypically Fra2-dependent. However, control of Cth2-target transcripts which is independent of CTH2 transcript abundance or of Fra2, is also quantified. Use of copper-BPQ supports a substantial contribution of metabolite repression to iron-regulation.
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Registered trials
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