ReviewThe Journal of membrane biology2014
Application of amphipols for structure-functional analysis of TRP channels.
Review in The Journal of membrane biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Discovery of an allosteric binding site for anthraquinones at the human P2X4 receptor.Nature communications · 2025Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Dawning of a new era in TRP channel structural biology by cryo-electron microscopy.Pflugers Archiv : European journal of physiology · 2018Review
- Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure.Biochimica et biophysica acta. Biomembranes · 2018Article
- Novobiocin, a Newly Found TRPV1 Inhibitor, Attenuates the Expression of TRPV1 in Rat Intestine and Intestinal Epithelial Cell Line IEC-6.Frontiers in pharmacology · 2018Article
- Flotillin scaffold activity contributes to type VII secretion system assembly in Staphylococcus aureus.PLoS pathogens · 2017Article
- Structure of the full-length TRPV2 channel by cryo-EM.Nature communications · 2016Article
- Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a.BMC biochemistry · 2015Article
- Synthesis, characterization and applications of a perdeuterated amphipol.The Journal of membrane biology · 2014Article
- Thermal fluctuations in amphipol A8-35 particles: a neutron scattering and molecular dynamics study.The Journal of membrane biology · 2014Article
- Amphipol-mediated screening of molecular orthoses specific for membrane protein targets.The Journal of membrane biology · 2014Article
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Isolation of Escherichia coli mannitol permease, EIImtl, trapped in amphipol A8-35 and fluorescein-labeled A8-35.The Journal of membrane biology · 2014Article
- Solution behavior and crystallization of cytochrome bc₁ in the presence of amphipols.The Journal of membrane biology · 2014Article
- Molecular dynamics simulations of a membrane protein/amphipol complex.The Journal of membrane biology · 2014Article
- Labeling and functionalizing amphipols for biological applications.The Journal of membrane biology · 2014Review
- How amphipols embed membrane proteins: global solvent accessibility and interaction with a flexible protein terminus.The Journal of membrane biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Amphipathic polymers (amphipols), such as A8-35 and SApol, are a new tool for stabilizing integral membrane proteins in detergent-free conditions for structural and functional studies. Transient receptor potential (TRP) ion channels function as tetrameric protein complexes in a diverse range of cellular processes including sensory transduction. Mammalian TRP channels share ~20 % sequence similarity and are categorized into six subfamilies: TRPC (canonical), TRPV (vanilloid), TRPA (ankyrin), TRPM (melastatin), TRPP (polycystin), and TRPML (mucolipin). Due to the inherent difficulties in purifying eukaryotic membrane proteins, structural studies of TRP channels have been limited. Recently, A8-35 was essential in resolving the molecular architecture of the nociceptor TRPA1 and led to the determination of a high-resolution structure of the thermosensitive TRPV1 channel by cryo-EM. Newly developed maltose-neopentyl glycol (MNG) detergents have also proven to be useful in stabilizing TRP channels for structural analysis. In this review, we will discuss the impacts of amphipols and MNG detergents on structural studies of TRP channels by cryo-EM. We will compare how A8-35 and MNG detergents interact with the hydrophobic transmembrane domains of TRP channels. In addition, we will discuss what these cryo-EM studies reveal on the importance of screening different types of surfactants toward determining high-resolution structures of TRP channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.