ArticlePurinergic signalling2014
Schistosome apyrase SmATPDase1, but not SmATPDase2, hydrolyses exogenous ATP and ADP.
Article in Purinergic signalling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Schistosoma mansoni and the purinergic halo.Trends in parasitology · 2022Review
- Identification of Asiaticoside fromPharmaceutics · 2022Article
- Mining Helminths for Novel Therapeutics.Trends in molecular medicine · 2021Review
- E-NTPDases: Possible Roles on Host-Parasite Interactions and Therapeutic Opportunities.Frontiers in cellular and infection microbiology · 2021Review
- Basic Coagulation Profiles and Platelet Count AmongResearch and reports in tropical medicine · 2020Article
- Why Do Intravascular Schistosomes Coat Themselves in Glycolytic Enzymes?BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- Gene Expression in Developmental Stages ofInternational journal of molecular sciences · 2019Article
- Purinergic receptors and neglected tropical diseases: why ignore purinergic signaling in the search for new molecular targets?Journal of bioenergetics and biomembranes · 2018Review
- The Essential Ectoenzyme SmNPP5 from the Human Intravascular Parasite Schistosoma mansoni is an ADPase and a Potent Inhibitor of Platelet Aggregation.Thrombosis and haemostasis · 2018Article
- A crosstalk between extracellular ATP and jasmonate signaling pathways for plant defense.Plant signaling & behavior · 2018Article
- IntravascularFrontiers in immunology · 2018Article
- Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide.Parasites & vectors · 2017Article
- Purinergic signaling in schistosomal infection.Biomedical journal · 2016Review
- Flavonoids and Sesquiterpene Lactones fromEvidence-based complementary and alternative medicine : eCAM · 2016Article
- Extracellular ATP acts as a damage-associated molecular pattern (DAMP) signal in plants.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Schistosomes are parasitic worms that can live in the bloodstream of their vertebrate hosts for many years. It has been proposed that the worms impinge on host purinergic signalling by degrading proinflammatory molecules like ATP as well as prothrombotic mediators like ADP. This capability may help explain the apparent refractoriness of the worms to both immune elimination and thrombus formation. Three distinct ectoenzymes, expressed at the host-exposed surface of the worm's tegument, are proposed to be involved in the catabolism of ATP and ADP. These are alkaline phosphatase (SmAP), phosphodiesterase (SmNPP-5), and ATP diphosphohydrolase (SmATPDase1). It has recently been shown that only one of these enzymes-SmATPDase1-actually degrades exogenous ATP and ADP. However, a second ATP diphosphohydrolase homolog (SmATPDase2) is located in the tegument and has been reported to be released by the worms. It is possible that this enzyme too participates in the cleavage of exogenous nucleotide tri- and di-phosphates. To test this hypothesis, we employed RNA interference (RNAi) to suppress the expression of the schistosome SmATPDase1 and SmATPDase2 genes. We find that only SmATPDase1-suppressed parasites are significantly impaired in their ability to degrade exogenously added ATP or ADP. Suppression of SmATPDase2 does not appreciably affect the worms' ability to catabolize ATP or ADP. Furthermore, we detect no evidence for the secretion or release of an ATP-hydrolyzing activity by cultured parasites. The results confirm the role of tegumental SmATPDase1, but not SmADTPDase2, in the degradation of the exogenous proinflammatory and prothrombotic nucleotides ATP and ADP by live intravascular stages of the parasite.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.