ArticleJournal of visualized experiments : JoVE2014
Two-photon in vivo imaging of dendritic spines in the mouse cortex using a thinned-skull preparation.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A model of ethanol self-administration in head-fixed mice.Alcohol, clinical & experimental research · 2025Article
- Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser.STAR protocols · 2022Article
- Disrupted Timing of MET Signaling Derails the Developmental Maturation of Cortical Circuits and Leads to Altered Behavior in Mice.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Stress induces microglia-associated synaptic circuit alterations in the dorsomedial prefrontal cortex.Neurobiology of stress · 2021Article
- Assessing Microglial Dynamics by Live Imaging.Frontiers in immunology · 2021Review
- Spatio-temporal dynamics of neocortical presynaptic terminal development using multi-photon imaging of the corpus callosum in vivo.Scientific reports · 2019Article
- Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution.Science (New York, N.Y.) · 2019Article
- Oncogenic activation of PI3K induces progenitor cell differentiation to suppress epidermal growth.Nature cell biology · 2018Article
- Postnatal Ablation of Synaptic Retinoic Acid Signaling Impairs Cortical Information Processing and Sensory Discrimination in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Skull optical clearing window forLight, science & applications · 2018Article
- Article
- Astrocytic Contributions to Synaptic and Learning Abnormalities in a Mouse Model of Fragile X Syndrome.Biological psychiatry · 2017Article
- Nutritional and Nanotechnological Modulators of Microglia.Frontiers in immunology · 2016Review
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Authors and funding
2 authors.
Funding
Abstract
In the mammalian cortex, neurons form extremely complicated networks and exchange information at synapses. Changes in synaptic strength, as well as addition/removal of synapses, occur in an experience-dependent manner, providing the structural foundation of neuronal plasticity. As postsynaptic components of the most excitatory synapses in the cortex, dendritic spines are considered to be a good proxy of synapses. Taking advantages of mouse genetics and fluorescent labeling techniques, individual neurons and their synaptic structures can be labeled in the intact brain. Here we introduce a transcranial imaging protocol using two-photon laser scanning microscopy to follow fluorescently labeled postsynaptic dendritic spines over time in vivo. This protocol utilizes a thinned-skull preparation, which keeps the skull intact and avoids inflammatory effects caused by exposure of the meninges and the cortex. Therefore, images can be acquired immediately after surgery is performed. The experimental procedure can be performed repetitively over various time intervals ranging from hours to years. The application of this preparation can also be expanded to investigate different cortical regions and layers, as well as other cell types, under physiological and pathological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.