ArticleeLife2014
Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Synergistic effect of PIPBiophysical journal · 2026Article
- Force Transmission by Minimal Focal Adhesion Complexes Induces Synthetic Cell Deformation.ACS synthetic biology · 2026Article
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- The role and regulation of integrins in cell migration and invasion.Nature reviews. Molecular cell biology · 2025Review
- The ubiquitous nanocluster: A molecular scale organizing principle that governs cellular information flow.Current opinion in cell biology · 2024Review
- Surface-induced phase separation of reconstituted nascent integrin clusters on lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events.Heliyon · 2023Article
- Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions.Nature reviews. Molecular cell biology · 2023Review
- Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation.eLife · 2022Article
- Article
- Article
- Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation.The Journal of cell biology · 2020Article
- Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds.Science advances · 2020Article
- Relief of talin autoinhibition triggers a force-independent association with vinculin.The Journal of cell biology · 2020Article
- The bioenergetics of integrin-based adhesion, from single molecule dynamics to stability of macromolecular complexes.Computational and structural biotechnology journal · 2020Review
- Regulation of Transmembrane Signaling by Phase Separation.Annual review of biophysics · 2019Review
- Cellular tension encodes local Src-dependent differential βMolecular biology of the cell · 2019Article
- Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions.The Journal of cell biology · 2018Article
- Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions.Scientific reports · 2018Article
- Molecular stretching modulates mechanosensing pathways.Protein science : a publication of the Protein Society · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How can the integrin adhesome get self-assembled locally, rapidly, and correctly as diverse cell-matrix adhesion sites? Here, we investigate this question by exploring the cytosolic state of integrin-adhesome components and their dynamic exchange between adhesion sites and cytosol. Using fluorescence cross-correlation spectroscopy (FCCS) and fluorescence recovery after photobleaching (FRAP) we found that the integrin adhesome is extensively pre-assembled already in the cytosol as multi-protein building blocks for adhesion sites. Stationary focal adhesions release symmetrically the same types of protein complexes that they recruit, thereby keeping the cytosolic pool of building blocks spatiotemporally uniform. We conclude a model in which multi-protein building blocks enable rapid and modular self-assembly of adhesion sites and symmetric exchange of these building blocks preserves their specifications and thus the assembly logic of the system.DOI: http://dx.doi.org/10.7554/eLife.02257.001.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.