ArticleJournal of visualized experiments : JoVE2014
Application of retinoic acid to obtain osteocytes cultures from primary mouse osteoblasts.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Targeted silencing ofFrontiers in endocrinology · 2024Article
- Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.JBMR plus · 2023Article
- Necroptosis: A new target for prevention of osteoporosis.Frontiers in endocrinology · 2022Review
- Comparison of Necroptosis With Apoptosis for OVX-Induced Osteoporosis.Frontiers in molecular biosciences · 2021Article
- Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.Stem cell research & therapy · 2020Article
- FGF23 and Fetuin-A Interaction and Mesenchymal Osteogenic Transformation.International journal of molecular sciences · 2019Article
- [Estradiol significantly increases the expression of antioxidant enzymes in osteoporotic rats and osteoblasts in vitro].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Article
- Retinoid receptors in bone and their role in bone remodeling.Frontiers in endocrinology · 2015Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The need for osteocyte cultures is well known to the community of bone researchers; isolation of primary osteocytes is difficult and produces low cell numbers. Therefore, the most widely used cellular system is the osteocyte-like MLO-Y4 cell line. The method here described refers to the use of retinoic acid to generate a homogeneous population of ramified cells with morphological and molecular osteocyte features. After isolation of osteoblasts from mouse calvaria, all-trans retinoic acid (ATRA) is added to cell medium, and cell monitoring is conducted daily under an inverted microscope. First morphological changes are detectable after 2 days of treatment and differentiation is generally complete in 5 days, with progressive development of dendrites, loss of the ability to produce extracellular matrix, down-regulation of osteoblast markers and up-regulation of osteocyte-specific molecules. Daily cell monitoring is needed because of the inherent variability of primary cells, and the protocol can be adapted with minimal variation to cells obtained from different mouse strains and applied to transgenic models. The method is easy to perform and does not require special instrumentation, it is highly reproducible, and rapidly generates a mature osteocyte population in complete absence of extracellular matrix, allowing the use of these cells for unlimited biological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.