ArticleJournal of visualized experiments : JoVE2014
Investigating protein-protein interactions in live cells using bioluminescence resonance energy transfer.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Functional methods for evaluating the efficacy of retinal optogenetic therapy for vision restoration.Frontiers in neuroscience · 2026Review
- Compensation between FOXP transcription factors maintains proper striatal function.Cell reports · 2024Article
- Compensation between FOXP transcription factors maintains proper striatal function.bioRxiv : the preprint server for biology · 2023Article
- Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions.Human molecular genetics · 2023Article
- Heterozygous variants that disturb the transcriptional repressor activity of FOXP4 cause a developmental disorder with speech/language delays and multiple congenital abnormalities.Genetics in medicine : official journal of the American College of Medical Genetics · 2021Article
- Article
- Mutations of the Transcriptional Corepressor ZMYM2 Cause Syndromic Urinary Tract Malformations.American journal of human genetics · 2020Article
- De Novo Variants Disturbing the Transactivation Capacity of POU3F3 Cause a Characteristic Neurodevelopmental Disorder.American journal of human genetics · 2019Article
- Functional characterization of TBR1 variants in neurodevelopmental disorder.Scientific reports · 2018Article
- Molecular Evidence of Adenosine Deaminase Linking Adenosine AFrontiers in pharmacology · 2018Article
- In Vivo Analysis of Protein-Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects.International journal of molecular sciences · 2016Review
- BCL11A Haploinsufficiency Causes an Intellectual Disability Syndrome and Dysregulates Transcription.American journal of human genetics · 2016Article
- The language-related transcription factor FOXP2 is post-translationally modified with small ubiquitin-like modifiers.Scientific reports · 2016Article
- Functional characterization of rare FOXP2 variants in neurodevelopmental disorder.Journal of neurodevelopmental disorders · 2016Article
- A de novo FOXP1 variant in a patient with autism, intellectual disability and severe speech and language impairment.European journal of human genetics : EJHG · 2015Article
- De novo TBR1 mutations in sporadic autism disrupt protein functions.Nature communications · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live cells. BRET is the non-radiative transfer of energy from a 'donor' luciferase enzyme to an 'acceptor' fluorescent protein. In the most common configuration of this assay, the donor is Renilla reniformis luciferase and the acceptor is Yellow Fluorescent Protein (YFP). Because the efficiency of energy transfer is strongly distance-dependent, observation of the BRET phenomenon requires that the donor and acceptor be in close proximity. To test for an interaction between two proteins of interest in cultured mammalian cells, one protein is expressed as a fusion with luciferase and the second as a fusion with YFP. An interaction between the two proteins of interest may bring the donor and acceptor sufficiently close for energy transfer to occur. Compared to other techniques for investigating protein-protein interactions, the BRET assay is sensitive, requires little hands-on time and few reagents, and is able to detect interactions which are weak, transient, or dependent on the biochemical environment found within a live cell. It is therefore an ideal approach for confirming putative interactions suggested by yeast two-hybrid or mass spectrometry proteomics studies, and in addition it is well-suited for mapping interacting regions, assessing the effect of post-translational modifications on protein-protein interactions, and evaluating the impact of mutations identified in patient DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.