ArticleJournal of visualized experiments : JoVE2014
Lineage-reprogramming of pericyte-derived cells of the adult human brain into induced neurons.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Spatiotemporal dynamics of brain pericytes in ischemic stroke.Journal of neuroinflammation · 2026Review
- Review
- Virtual Screening-Based Drug Development for the Treatment of Nervous System Diseases.Current neuropharmacology · 2023Review
- Cell Reprogramming for Regeneration and Repair of the Nervous System.Biomedicines · 2022Review
- Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering.ACS omega · 2021Article
- Neuroregeneration and functional recovery after stroke: advancing neural stem cell therapy toward clinical application.Neural regeneration research · 2021Article
- Pericytes in Microvessels: From "Mural" Function to Brain and Retina Regeneration.International journal of molecular sciences · 2019Review
- Targeting pericytes for therapeutic approaches to neurological disorders.Acta neuropathologica · 2018Review
- Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program.Nature neuroscience · 2018Article
- Direct Conversion of Somatic Cells into Induced Neurons.Molecular neurobiology · 2018Review
- Pericytes in Brain Injury and Repair After Ischemic Stroke.Translational stroke research · 2017Review
- Induced dopaminergic neurons: A new promise for Parkinson's disease.Redox biology · 2017Article
- PBX1 as Pioneer Factor: A Case Still Open.Frontiers in cell and developmental biology · 2017Review
- Perivascular Mesenchymal Stem Cells From the Adult Human Brain Harbor No Instrinsic Neuroectodermal but High Mesodermal Differentiation Potential.Stem cells translational medicine · 2015Article
- Stem cell therapies for age-related macular degeneration: the past, present, and future.Clinical interventions in aging · 2015Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct lineage-reprogramming of non-neuronal cells into induced neurons (iNs) may provide insights into the molecular mechanisms underlying neurogenesis and enable new strategies for in vitro modeling or repairing the diseased brain. Identifying brain-resident non-neuronal cell types amenable to direct conversion into iNs might allow for launching such an approach in situ, i.e. within the damaged brain tissue. Here we describe a protocol developed in the attempt of identifying cells derived from the adult human brain that fulfill this premise. This protocol involves: (1) the culturing of human cells from the cerebral cortex obtained from adult human brain biopsies; (2) the in vitro expansion (approximately requiring 2-4 weeks) and characterization of the culture by immunocytochemistry and flow cytometry; (3) the enrichment by fluorescence-activated cell sorting (FACS) using anti-PDGF receptor-β and anti-CD146 antibodies; (4) the retrovirus-mediated transduction with the neurogenic transcription factors sox2 and ascl1; (5) and finally the characterization of the resultant pericyte-derived induced neurons (PdiNs) by immunocytochemistry (14 days to 8 weeks following retroviral transduction). At this stage, iNs can be probed for their electrical properties by patch-clamp recording. This protocol provides a highly reproducible procedure for the in vitro lineage conversion of brain-resident pericytes into functional human iNs.
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