ArticleMarine drugs2014
Metabolomic profiling and genomic study of a marine sponge-associated Streptomyces sp.
Article in Marine drugs, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Defining the biomarkers in anti-MRSA fractions of soil Streptomycetes by multivariate analysis.Antonie van Leeuwenhoek · 2025Article
- Gut and Gill-Associated Microbiota of the Flatfish European Plaice (Marine drugs · 2022Article
- Indispensable role of microbes in anticancer drugs and discovery trends.Applied microbiology and biotechnology · 2022Review
- Metabolomic Profiling and Molecular Networking of Nudibranch-AssociatedMolecules (Basel, Switzerland) · 2022Article
- Metabolomics on the study of marine organisms.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- Comparative metabolomic analysis reveals shared and unique chemical interactions in sponge holobionts.Microbiome · 2022Article
- A NewMarine drugs · 2021Article
- Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge IsolateMicroorganisms · 2019Article
- Marine Natural Products: A Source of Novel Anticancer Drugs.Marine drugs · 2019Review
- Regulation of Antibiotic Production by Signaling Molecules inFrontiers in microbiology · 2019Review
- Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt.PloS one · 2019Article
- Butenolides from Streptomyces albus J1074 Act as External Signals To Stimulate Avermectin Production in Streptomyces avermitilis.Applied and environmental microbiology · 2018Article
- Draft Genome Sequence of the Antimycin-Producing BacteriumGenome announcements · 2018Article
- Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074.Scientific reports · 2017Article
- Current Status and Future Prospects of Marine Natural Products (MNPs) as Antimicrobials.Marine drugs · 2017Review
- Article
- Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins.Journal of industrial microbiology & biotechnology · 2016Article
- Bioprospecting Sponge-Associated Microbes for Antimicrobial Compounds.Marine drugs · 2016Review
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolomics and genomics are two complementary platforms for analyzing an organism as they provide information on the phenotype and genotype, respectively. These two techniques were applied in the dereplication and identification of bioactive compounds from a Streptomyces sp. (SM8) isolated from the sponge Haliclona simulans from Irish waters. Streptomyces strain SM8 extracts showed antibacterial and antifungal activity. NMR analysis of the active fractions proved that hydroxylated saturated fatty acids were the major components present in the antibacterial fractions. Antimycin compounds were initially putatively identified in the antifungal fractions using LC-Orbitrap. Their presence was later confirmed by comparison to a standard. Genomic analysis of Streptomyces sp. SM8 revealed the presence of multiple secondary metabolism gene clusters, including a gene cluster for the biosynthesis of the antifungal antimycin family of compounds. The antimycin gene cluster of Streptomyces sp. SM8 was inactivated by disruption of the antimycin biosynthesis gene antC. Extracts from this mutant strain showed loss of antimycin production and significantly less antifungal activity than the wild-type strain. Three butenolides, 4,10-dihydroxy-10-methyl-dodec-2-en-1,4-olide (1), 4,11-dihydroxy-10-methyl-dodec-2-en-1,4-olide (2), and 4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide (3) that had previously been reported from marine Streptomyces species were also isolated from SM8. Comparison of the extracts of Streptomyces strain SM8 and its host sponge, H. simulans, using LC-Orbitrap revealed the presence of metabolites common to both extracts, providing direct evidence linking sponge metabolites to a specific microbial symbiont.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.