Evidence map›Paper›PMID 24893291›Full record

ArticlePloS one2014

Timed deletion of Twist1 in the limb bud reveals age-specific impacts on autopod and zeugopod patterning.

David A F Loebel, Angelyn C C Hor, Heidi K Bildsoe, Patrick P L Tam

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. The two domain hypothesis of limb prepattern and its relevance to congenital limb anomalies.Wiley interdisciplinary reviews. Developmental biology · 2017
    Review
  2. Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

David A F LoebelEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales, Australia; Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
Angelyn C C HorEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales, Australia.
Heidi K BildsoeEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales, Australia; Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
Patrick P L TamEmbryology Unit, Children's Medical Research Institute, Westmead, New South Wales, Australia; Sydney Medical School, The University of Sydney, Sydney, New South Wales, Australia.
Children's Medical Research Institute · AUUniversity of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Twist1 encodes a transcription factor that plays a vital role in limb development. We have used a tamoxifen-inducible Cre transgene, Ubc-CreERT2, to generate time-specific deletions of Twist1 by inducing Cre activity in mouse embryos at different ages from embryonic (E) day 9.5 onwards. A novel forelimb phenotype of supernumerary pre-axial digits and enlargement or partial duplication of the distal radius was observed when Cre activity was induced at E9.5. Gene expression analysis revealed significant upregulation of Hoxd10, Hoxd11 and Grem1 in the anterior half of the forelimb bud at E11.5. There is also localized upregulation of Ptch1, Hand2 and Hoxd13 at the site of ectopic digit formation, indicating a posterior molecular identity for the supernumerary digits. The specific skeletal phenotypes, which include duplication of digits and distal zeugopods but no overt posteriorization, differ from those of other Twist1 conditional knockout mutants. This outcome may be attributed to the deferment of Twist1 ablation to a later time frame of limb morphogenesis, which leads to the ectopic activation of posterior genes in the anterior tissues after the establishment of anterior-posterior anatomical identities in the forelimb bud.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsBody PatterningCytokinesEmbryo, MammalianEmbryonic DevelopmentFemaleForelimbGene Expression Regulation, DevelopmentalHomeodomain ProteinsIntercellular Signaling Peptides and ProteinsLimb BudsMiceMice, KnockoutNuclear ProteinsPatched-1 ReceptorBasic Helix-Loop-Helix ProteinsCytokinesGrem1 protein, mouseHand2 protein, mouseHomeodomain ProteinsHoxd10 protein, mouseHoxd13 protein, mouseHoxd1 protein, mouseIntercellular Signaling Peptides and ProteinsNuclear ProteinsPatched-1 ReceptorPatched ReceptorsPtch1 protein, mouseReceptors, Cell SurfaceTranscription FactorsTwist1 protein, mouseTwist-Related Protein 1

Identifiers

PMID24893291
PMCPMC4044014
OpenAlexW2065797568

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.