ArticlePloS one2014
Overexpression of phosphomimic mutated OsWRKY53 leads to enhanced blast resistance in rice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 131 citations in OpenAlex.
- Article
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- Functional and regulatory complexity: challenges and prospects for understanding rice WRKY transcription factors.Journal of experimental botany · 2026Review
- A SERK3-PSKR1 Module Negatively Regulates Rice Resistance to Planthoppers.Plant, cell & environment · 2026Article
- OsMAPK6 Phosphorylates and Degrades OsCATA to Regulate HMolecular plant pathology · 2026Article
- Rice roots recruit Bacillus via the secretion of heptadecanoic acid.Nature plants · 2026Article
- FAD-Linked Oxidoreductase Protein 1 (FLO1) Coordinates Grain Development and Drought Tolerance in Rice.Plants (Basel, Switzerland) · 2026Article
- Engineering resilient roses: molecular insights into biotic and abiotic stress adaptation.Horticulture research · 2026Article
- Article
- Recent advances in improving yield and immunity through transcription factor engineering.Journal of integrative plant biology · 2025Review
- Integrative multi-transcriptomic analysis uncovers core genes and potential defense mechanisms in rice-Magnoporthe oryzae interaction.Plant cell reports · 2025Article
- The OsMAPK6-OsWRKY72 module positively regulates rice leaf angle through brassinosteroid signals.Plant communications · 2025Article
- Enhancing Crop Resilience: Insights from Labdane-Related Diterpenoid Phytoalexin Research in Rice (Current issues in molecular biology · 2024Review
- Post-Translational Modification of WRKY Transcription Factors.Plants (Basel, Switzerland) · 2024Review
- Deletion of the sugar importer gene OsSWEET1b accelerates sugar starvation-promoted leaf senescence in rice.Plant physiology · 2024Article
- OsMAPK6 phosphorylation and CLG1 ubiquitylation of GW6a non-additively enhance rice grain size through stabilization of the substrate.Nature communications · 2024Article
- The GRAS protein OsDLA involves in brassinosteroid signalling and positively regulates blast resistance by forming a module with GSK2 and OsWRKY53 in rice.Plant biotechnology journal · 2024Article
- OsWRKY26 negatively regulates bacterial blight resistance by suppressingFrontiers in plant science · 2024Article
- Recent advances in functional assays of WRKY transcription factors in plant immunity against pathogens.Frontiers in plant science · 2024Review
- Rice breeding for low input agriculture.Frontiers in plant science · 2024Review
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.
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