ReviewIndian journal of microbiology2014
Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP).
Review in Indian journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Lactic acid bacteria as an eco-friendly approach in plant production: Current state and prospects.Folia microbiologica · 2024Review
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
- Design and validation of Dolosigranulum pigrum specific PCR primers using the bacterial core genome.Scientific reports · 2023Article
- Prospects of advanced metagenomics and meta-omics in the investigation of phytomicrobiome to forecast beneficial and pathogenic response.Molecular biology reports · 2022Review
- High-Throughput Sequencing and Unsupervised Analysis of Formyltetrahydrofolate Synthetase (FTHFS) Gene Amplicons to Estimate Acetogenic Community Structure.Frontiers in microbiology · 2020Article
- Experimental and computational approaches to unravel microbial community assembly.Computational and structural biotechnology journal · 2020Review
- Terminal restriction fragment length polymorphism is an "old school" reliable technique for swift microbial community screening in anaerobic digestion.Scientific reports · 2018Article
- A comparison study of the potential risks induced in arable land and forest soils by carcass-derived pollutants.Environmental geochemistry and health · 2018Article
- Article
- Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase.Scientific reports · 2017Article
- Genome Wide Analysis for Searching Novel Markers to Rapidly Identify Clostridium Strains.Indian journal of microbiology · 2015Article
- Applications of Next-generation Sequencing in Systemic Autoimmune Diseases.Genomics, proteomics & bioinformatics · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Terminal restriction fragment length polymorphism (T-RFLP) is a rapid, robust, inexpensive and simple tool for microbial community profiling. Methods used for DNA extraction, PCR amplification and digestion of amplified products have a considerable impact on the results of T-RFLP. Pitfalls of the method skew the similarity analysis and compromise its high throughput ability. Despite a high throughput method of data generation, data analysis is still in its infancy and needs more attention. Current article highlights the limitations of the methods used for data generation and analysis. It also provides an overview of the recent methodological developments in T-RFLP which will assist the readers in obtaining real and authentic profiles of the microbial communities under consideration while eluding the inherent biases and technical difficulties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.