ArticleJournal of lipid research2014
Enhanced lipid isomer separation in human plasma using reversed-phase UPLC with ion-mobility/high-resolution MS detection.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- De novo lipogenesis is essential for platelet production in humans.Nature metabolism · 2020Trial
- Application of Ion Mobility Mass Spectrometry in Lipidomics.Advances in experimental medicine and biology · 2026Review
- Comprehensive analysis through multi-omics integration to compare and elucidate the specific substances in milk of donkey、horse、camel、human and pig.Food chemistry: X · 2025Article
- Advancements in Mass Spectrometry-Based Targeted Metabolomics and Lipidomics: Implications for Clinical Research.Molecules (Basel, Switzerland) · 2024Review
- Unraveling the complexity of glycosphingolipidome: the key role of mass spectrometry in the structural analysis of glycosphingolipids.Analytical and bioanalytical chemistry · 2024Review
- LC-ESI-HRMS - lipidomics of phospholipids : Characterization of extraction, chromatography and detection parameters.Analytical and bioanalytical chemistry · 2024Article
- Utilization of LC-MS/MS and Drift Tube Ion Mobility for Characterizing Intact Oxidized Arachidonate-Containing Glycerophosphatidylethanolamine.Journal of the American Society for Mass Spectrometry · 2023Article
- Systematic untargeted UHPLC-Q-TOF-MS based lipidomics workflow for improved detection and annotation of lipid sub-classes in serum.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- Integrating the potential of ion mobility spectrometry-mass spectrometry in the separation and structural characterisation of lipid isomers.Frontiers in molecular biosciences · 2023Review
- Linoleic acid metabolism activation in macrophages promotes the clearing of intracellularChemical science · 2022Article
- Profiling Phospholipids within Atlantic SalmonMetabolites · 2022Article
- A reversed phase ultra-high-performance liquid chromatography-data independent mass spectrometry method for the rapid identification of mycobacterial lipids.Journal of chromatography. A · 2022Article
- Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS.Analytical and bioanalytical chemistry · 2022Article
- Phosphatidic acid phosphatase 1 impairs SARS-CoV-2 replication by affecting the glycerophospholipid metabolism pathway.International journal of biological sciences · 2022Article
- Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS.Journal of the American Society for Mass Spectrometry · 2021Article
- Age-related susceptibility to insulin resistance arises from a combination of CPT1B decline and lipid overload.BMC biology · 2021Article
- Triboelectric Nanogenerator Ion Mobility-Mass Spectrometry for In-Depth Lipid Annotation.Analytical chemistry · 2021Article
- A Novel Approach to Characterize the Lipidome of Marine ArchaeonFrontiers in microbiology · 2021Article
- Functional Metabolomics and Chemoproteomics Approaches Reveal Novel Metabolic Targets for Anticancer Therapy.Advances in experimental medicine and biology · 2021Article
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5 authors.
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Abstract
An ultraperformance LC (UPLC) method for the separation of different lipid molecular species and lipid isomers using a stationary phase incorporating charged surface hybrid (CSH) technology is described. The resulting enhanced separation possibilities of the method are demonstrated using standards and human plasma extracts. Lipids were extracted from human plasma samples with the Bligh and Dyer method. Separation of lipids was achieved on a 100 × 2.1 mm inner diameter CSH C18 column using gradient elution with aqueous-acetonitrile-isopropanol mobile phases containing 10 mM ammonium formate/0.1% formic acid buffers at a flow rate of 0.4 ml/min. A UPLC run time of 20 min was routinely used, and a shorter method with a 10 min run time is also described. The method shows extremely stable retention times when human plasma extracts and a variety of biofluids or tissues are analyzed [intra-assay relative standard deviation (RSD) <0.385% and <0.451% for 20 and 10 min gradients, respectively (n = 5); interassay RSD <0.673% and <0.763% for 20 and 10 min gradients, respectively (n = 30)]. The UPLC system was coupled to a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer, equipped with a traveling wave ion-mobility cell. Besides demonstrating the separation for different lipids using the chromatographic method, we demonstrate the use of the ion-mobility MS platform for the structural elucidation of lipids. The method can now be used to elucidate structures of a wide variety of lipids in biological samples of different matrices.
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