Evidence map›Paper›PMID 24891284›Full record

ArticleCroatian medical journal2014

Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings.

Yuriy Kit, Marina Starykovych, Marie Vajrychova, Juraj Lenco, Danuta Zastavna, Rostyslav Stoika

Open access · diamondAbstract read
In one paragraph

Article in Croatian medical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Yuriy Kit
Marina Starykovych
Marie Vajrychova
Juraj Lenco
Danuta Zastavna
Rostyslav StoikaRostyslav Stoika, Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005, Lviv, Ukraine, stoika@cellbiol.lviv.ua.
National Academy of Sciences of Ukraine · UAUniversity of Defence · CZInstitute of Cell Biology · UANational Academy of Medical Sciences of Ukraine · UA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM).

methodsThe strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification.

resultsFive potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2.

conclusionsWe developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value.

Indexed as

Abortion, HabitualActinsAdenosine TriphosphatasesAdultalpha-GlucosidasesAutoantibodiesAutoantigensBlotting, WesternChorionChromatography, AffinityEgg ProteinsElectrophoresis, Polyacrylamide GelEndoplasmic ReticulumFemaleHumansMembrane GlycoproteinsActinsAdenosine Triphosphatasesalpha-GlucosidasesAutoantibodiesAutoantigenschorion proteinsEgg ProteinsendoplasminMembrane Glycoproteins

Identifiers

PMID24891284
PMCPMC4049207
OpenAlexW1988333614

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.