ArticleChembiochem : a European journal of chemical biology2014
Exploring the substrate range of wild-type aminoacyl-tRNA synthetases.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- tRNA shape is an identity element for an archaeal pyrrolysyl-tRNA synthetase from the human gut.Nucleic acids research · 2024Article
- Rational design of the genetic code expansion toolkit forFrontiers in genetics · 2023Article
- Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases.Chembiochem : a European journal of chemical biology · 2022Review
- Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway.Nature communications · 2021Article
- A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria.Nucleic acids research · 2021Article
- Ferritin Conjugates With Multiple Clickable Amino Acids Encoded by C-Terminal Engineered Pyrrolysyl-tRNA Synthetase.Frontiers in chemistry · 2021Article
- Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids.Molecules (Basel, Switzerland) · 2020Article
- Engineering aminoacyl-tRNA synthetases for use in synthetic biology.The Enzymes · 2020Article
- HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes.Chemical science · 2019Article
- Plasticity and Constraints of tRNA Aminoacylation Define Directed Evolution of Aminoacyl-tRNA Synthetases.International journal of molecular sciences · 2019Review
- Incorporation of Modified Amino Acids by Engineered Elongation Factors with Expanded Substrate Capabilities.ACS synthetic biology · 2019Article
- In vitro genetic code reprogramming and expansion to study protein function and discover macrocyclic peptide ligands.Current opinion in chemical biology · 2018Review
- Upgrading aminoacyl-tRNA synthetases for genetic code expansion.Current opinion in chemical biology · 2018Review
- Engineering posttranslational proofreading to discriminate nonstandard amino acids.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase.Nature chemical biology · 2017Article
- Rewiring protein synthesis: From natural to synthetic amino acids.Biochimica et biophysica acta. General subjects · 2017Review
- Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis.Biochimica et biophysica acta. General subjects · 2017Article
- A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase•tRNABiochimica et biophysica acta. General subjects · 2017Article
- Rewriting the Genetic Code.Annual review of microbiology · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We tested the substrate range of four wild-type E. coli aminoacyl-tRNA synthetases (AARSs) with a library of nonstandard amino acids (nsAAs). Although these AARSs could discriminate efficiently against the other canonical amino acids, they were able to use many nsAAs as substrates. Our results also show that E. coli tryptophanyl-tRNA synthetase (TrpRS) and tyrosyl-tRNA synthetase have overlapping substrate ranges. In addition, we found that the nature of the anticodon sequence of tRNA(Trp) altered the nsAA substrate range of TrpRS; this implies that the sequence of the anticodon affects the TrpRS amino acid binding pocket. These results highlight again that inherent AARS polyspecificity will be a major challenge in the aim of incorporating multiple different amino acids site-specifically into proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.