Evidence map›Paper›PMID 24890370›Full record

ArticleParasite (Paris, France)2014

Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major.

Brianna Norris-Mullins, Paola Vacchina, Miguel A Morales

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Brianna Norris-MullinsDepartment of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
Paola VacchinaDepartment of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.
Miguel A MoralesDepartment of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed auto-inhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic intervention.

Indexed as

Amino Acid MotifsAniline CompoundsCatalysisEnzyme ActivationGenes, ProtozoanLeishmania majorMutagenesis, Site-DirectedNuclear ProteinsOrganophosphorus CompoundsPhosphoprotein PhosphatasesProtein Structure, TertiaryProtozoan ProteinsRecombinant Fusion ProteinsSequence AlignmentSequence Homology, Amino AcidSpecies Specificity4-aminophenylphosphateAniline CompoundsNuclear ProteinsOrganophosphorus CompoundsPhosphoprotein Phosphatasesprotein phosphatase 5Protozoan ProteinsRecombinant Fusion Proteins

Identifiers

PMID24890370
PMCPMC4042446

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.