ArticleAlcoholism, clinical and experimental research2014
Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays.
Article in Alcoholism, clinical and experimental research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- More than meets the eye: mutation of the white gene in Drosophila has broad phenotypic and transcriptomic effects.Genetics · 2025Article
- The function of ethanol in olfactory associative behaviors in Drosophila melanogaster larvae.PloS one · 2023Article
- Identification of additional dye tracers for measuring solid food intake and food preference via consumption-excretion in Drosophila.Scientific reports · 2022Article
- Chloride intracellular channel 4 (CLIC4) expression profile in the mouse medial prefrontal cortex and its regulation by ethanol.Alcoholism, clinical and experimental research · 2022Article
- Corazonin Neurons Contribute to Dimorphic Ethanol Sedation Sensitivity inFrontiers in neural circuits · 2022Article
- Mutants of theInternational journal of molecular sciences · 2021Article
- Expansion and application of dye tracers for measuring solid food intake and food preference in Drosophila.Scientific reports · 2021Article
- Tyramine synthesis, vesicular packaging, and the SNARE complex function coordinately in astrocytes to regulate Drosophila alcohol sedation.Addiction biology · 2021Article
- Article
- Serotoninergic Modulation of Phototactic Variability Underpins a Bet-Hedging Strategy inFrontiers in behavioral neuroscience · 2021Article
- Flying Together:International journal of molecular sciences · 2020Review
- Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function.Addiction biology · 2020Article
- Pharmacological targeting of the GABAAddiction biology · 2020Article
- Convergent Evidence From Humans and Drosophila melanogaster Implicates the Transcription Factor MEF2B/Mef2 in Alcohol Sensitivity.Alcoholism, clinical and experimental research · 2019Article
- Automated real-time quantification of group locomotor activity in Drosophila melanogaster.Scientific reports · 2019Article
- Alcohol sedation in adultCommunications biology · 2019Article
- Cross-species molecular dissection across alcohol behavioral domains.Alcohol (Fayetteville, N.Y.) · 2018Review
- Biogenesis of zinc storage granules inThe Journal of experimental biology · 2018Article
- Genetics and genomics of alcohol responses in Drosophila.Neuropharmacology · 2017Review
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9 authors at 2 institutions in 1 country.
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Abstract
backgroundThe fruit fly Drosophila melanogaster has been used extensively to investigate genetic mechanisms of ethanol (EtOH)-related behaviors. Many past studies in flies, including studies from our laboratory, have manipulated gene expression using transposons carrying the genetic-phenotypic marker mini-white(mini-w), a derivative of the endogenous gene white(w). Whether the mini-w transgenic marker or the endogenous w gene influences behavioral responses to acute EtOH exposure in flies has not been systematically investigated.
methodsWe manipulated mini-w and w expression via (i) transposons marked with mini-w, (ii) RNAi against mini-w and w, and (iii) a null allele of w. We assessed EtOH sensitivity and tolerance using a previously described eRING assay (based on climbing in the presence of EtOH) and an assay based on EtOH-induced sedation.
resultsIn eRING assays, EtOH-induced impairment of climbing correlated inversely with expression of the mini-w marker from a series of transposon insertions. Additionally, flies harboring a null allele of w or flies with RNAi-mediated knockdown of mini-w were significantly more sensitive to EtOH in eRING assays than controls expressing endogenous w or the mini-w marker. In contrast, EtOH sensitivity and rapid tolerance measured in the EtOH sedation assay were not affected by decreased expression of mini-w or endogenous w in flies.
conclusionsEtOH sensitivity measured in the eRING assay is noticeably influenced by w and mini-w, making eRING problematic for studies on EtOH-related behavior in Drosophila using transgenes marked with mini-w. In contrast, the EtOH sensitivity assay described here is a suitable behavioral paradigm for studies on EtOH sensitivity and rapid tolerance in Drosophila including those that use widely available transgenes marked with mini-w.
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