ArticleBMC research notes2014
Leptospira species and serovars identified by MALDI-TOF mass spectrometry after database implementation.
Article in BMC research notes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 29 citations in OpenAlex.
- Diagnosis of leptospirosis in animals: challenges and perspectives.Journal of clinical microbiology · 2026Review
- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Beyond serology: saccharide profiling enables identification of antigenically similarFrontiers in molecular biosciences · 2025Article
- MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory.Microorganisms · 2024Review
- First Report onMicroorganisms · 2023Article
- Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains ofMolecules (Basel, Switzerland) · 2022Article
- Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR.PLoS neglected tropical diseases · 2019Article
- Biodiversity of EnvironmentalFrontiers in microbiology · 2018Article
- MALDI-TOF mass spectrometry as a potential tool for Trichomonas vaginalis identification.BMC infectious diseases · 2016Article
- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists.Frontiers in microbiology · 2016Review
- Detection of Rickettsia spp in ticks by MALDI-TOF MS.PLoS neglected tropical diseases · 2015Article
- MALDI-TOF mass spectrometry for the detection and differentiation of Entamoeba histolytica and Entamoeba dispar.PloS one · 2015Article
- Identification of Dermatophyte species after implementation of the in-house MALDI-TOF MS database.International journal of molecular sciences · 2014Article
- Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospirosis, a spirochaetal zoonotic disease of worldwide distribution, endemic in Europe, has been recognized as an important emerging infectious disease, though yet it is mostly a neglected disease which imparts its greatest burden on impoverished populations from developing countries. Leptospirosis is caused by the infection with any of the more than 230 serovars of pathogenic Leptospira sp. In this study we aimed to implement the MALDI-TOF mass spectrometry (MS) database currently available in our laboratory with Leptospira reference pathogenic (L. interrogans, L. borgpetersenii, L. kirschneri, L. noguchii), intermediate (L. fainei) and saprophytic (L. biflexa) strains of our collection in order to evaluate its possible application to the diagnosis of leptospirosis and to the typing of strains. This was done with the goal of understanding whether this methodology could be used as a tool for the identification of Leptospira strains, not only at species level for diagnostic purposes, but also at serovar level for epidemiological purposes, overcoming the limits of serological and molecular conventional methods. Twenty Leptospira reference strains were analysed by MALDI-TOF MS. Statistical analysis of the protein spectra was performed by ClinProTools software.
resultsThe spectra obtained by the analysis of the reference strains tested were grouped into 6 main classes corresponding to the species analysed, highlighting species-specific protein profiles. Moreover, the statistical analysis of the spectra identified discriminatory peaks to recognize Leptospira strains also at serovar level extending previously published data.
conclusionsIn conclusion, we confirmed that MALDI-TOF MS could be a powerful tool for research and diagnostic in the field of leptospirosis with broad applications ranging from the detection and identification of pathogenic leptospires for diagnostic purposes to the typing of pathogenic and non-pathogenic leptospires for epidemiological purposes in order to enrich our knowledge about the epidemiology of the infection in different areas and generate control strategies.
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