ArticlePloS one2014
The gluconeogenesis pathway is involved in maintenance of enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Metabolic strategies of Enterobacteriaceae gut colonization.Nature reviews. Microbiology · 2026Review
- Exometabolomic-enabled discovery of compounds associated with Escherichia coli O157:H7 population dynamics in the lettuce phyllosphere.BMC plant biology · 2026Article
- Transcriptomic profiling of a commensal Escherichia coli lysogen in simulated intestinal fluid reveals broad changes in both core and accessory regions of the genome.BMC genomics · 2026Article
- In Vitro Investigation of the Effects ofAnimals : an open access journal from MDPI · 2025Article
- Nutrition ofEcoSal Plus · 2024Review
- Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine.BMC genomics · 2022Article
- Transcriptional analysis reveals specific niche factors and response to environmental stresses of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents.BMC microbiology · 2021Article
- Diversity of the Tryptophanase Gene and Its Evolutionary Implications in Living Organisms.Microorganisms · 2021Article
- Disruption of the MreB Elongasome Is Overcome by Mutations in the Tricarboxylic Acid Cycle.Frontiers in microbiology · 2021Article
- Gut microbiome signatures of nursing home residents carrying Enterobacteria producing extended-spectrum β-lactamases.Antimicrobial resistance and infection control · 2020Article
- An Overview of the Elusive Passenger in the Gastrointestinal Tract of Cattle: The Shiga Toxin ProducingMicroorganisms · 2020Review
- Gut Microbiota and Colonization Resistance against Bacterial Enteric Infection.Microbiology and molecular biology reviews : MMBR · 2019Review
- Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents.BMC genomics · 2018Article
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- Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome.PloS one · 2018Article
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- The NAG Sensor NagC Regulates LEE Gene Expression and Contributes to Gut Colonization byFrontiers in cellular and infection microbiology · 2017Article
- Lactobacillus reuteri suppresses E. coli O157:H7 in bovine ruminal fluid: Toward a pre-slaughter strategy to improve food safety?PloS one · 2017Article
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Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) are responsible for outbreaks of food- and water-borne illness. The bovine gastrointestinal tract (GIT) is thought to be the principle reservoir of EHEC. Knowledge of the nutrients essential for EHEC growth and survival in the bovine intestine may help in developing strategies to limit their shedding in bovine faeces thus reducing the risk of human illnesses. To identify specific metabolic pathways induced in the animal GIT, the transcriptome profiles of EHEC O157:H7 EDL933 during incubation in bovine small intestine contents (BSIC) and minimal medium supplemented with glucose were compared. The transcriptome analysis revealed that genes responsible for the assimilation of ethanolamine, urea, agmatine and amino acids (Asp, Thr, Gly, Ser and Trp) were strongly up-regulated suggesting that these compounds are the main nitrogen sources for EHEC in BSIC. A central role for the gluconeogenesis pathway and assimilation of gluconeogenic substrates was also pinpointed in EHEC incubated in BSIC. Our results suggested that three amino acids (Asp, Ser and Trp), glycerol, glycerol 3-phosphate, L-lactate and C4-dicarboxylates are important carbon sources for EHEC in BSIC. The ability to use gluconeogenic substrates as nitrogen sources (amino acids) and/or carbon sources (amino acids, glycerol and lactate) may provide a growth advantage to the bacteria in intestinal fluids. Accordingly, aspartate (2.4 mM), serine (1.9 mM), glycerol (5.8 mM) and lactate (3.6 mM) were present in BSIC and may represent the main gluconeogenic substrates potentially used by EHEC. A double mutant of E. coli EDL933 defective for phosphoenolpyruvate synthase (PpsA) and phosphoenolpyruvate carboxykinase (PckA), unable to utilize tricarboxylic acid (TCA) intermediates was constructed. Growth competition experiments between EHEC EDL933 and the isogenic mutant strain in BSIC clearly showed a significant competitive growth advantage of the wild-type strain further illustrating the importance of the gluconeogenesis pathway in maintaining EHEC in the bovine GIT.
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