ArticleAoB PLANTS2014
Reprogramming of cassava (Manihot esculenta) microspores towards sporophytic development.
Article in AoB PLANTS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 17 citations in OpenAlex.
- A Scalable Droplet Microfluidic Platform That Overcomes Large-Cell Constraints for Long-Term Plant Cell Culture and Embryogenesis Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Defining the Optimal Microspore Developmental Window for Efficient Anther-Derived Somatic Embryogenesis in Rubber Tree (Plants (Basel, Switzerland) · 2026Article
- Association of morphological markers of flower buds and anthers with the pollen developmental stage inFrontiers in plant science · 2026Article
- Early Development of Direct Embryos in the Cultured Anthers ofPlants (Basel, Switzerland) · 2020Article
- Studies on Gynogenesis Induction in Cassava (Frontiers in plant science · 2020Article
- Protocol for Rescuing Young Cassava Embryos.Frontiers in plant science · 2020Article
- Decrypting tubby-like protein gene family of multiple functions in starch root crop cassava.AoB PLANTS · 2019Article
- Phylogeny and expression pattern analysis of TCP transcription factors in cassava seedlings exposed to cold and/or drought stress.Scientific reports · 2017Article
- The ERF transcription factor family in cassava: genome-wide characterization and expression analyses against drought stress.Scientific reports · 2016Article
- Genome-wide characterization and analysis of bZIP transcription factor gene family related to abiotic stress in cassava.Scientific reports · 2016Article
- Genome-Wide Identification and Expression Analysis of the Mitogen-Activated Protein Kinase Gene Family in Cassava.Frontiers in plant science · 2016Article
- Genome-Wide Identification and Expression Analysis of the WRKY Gene Family in Cassava.Frontiers in plant science · 2016Article
- Genome-Wide Identification and Expression Analysis of the NAC Transcription Factor Family in Cassava.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gametes have the unique potential to enter the sporophytic pathway, called androgenesis. The plants produced are usually haploid and recombinant due to the preceding meiosis and they can double their chromosome number to form doubled haploids, which are completely homozygous. Availability of the doubled haploids facilitates mapping the genes of agronomically important traits, shortening the time of the breeding process required to produce new hybrids and homozygous varieties, and saving the time and cost for inbreeding. This study aimed to test the feasibility of using isolated and in vitro cultured immature cassava (Manihot esculenta) microspores to reprogramme and initiate sporophytic development. Different culture media and different concentrations of two ion components (Cu(2+) and Fe(2+)) were tested in two genotypes of cassava. External structural changes, nuclear divisions and cellular changes during reprogramming were analysed by scanning electron microscopy, by staining with 4',6-diamidino-2-phenylindole, and through classical histology and transmission electron microscopy. In two cassava genotypes, different developmental stages of microspores were found to initiate sporophytic cell divisions, that is, with tetrads of TMS 60444 and with mid or late uni-nucleate microspores of SM 1219-9. In the modified NLN medium (NLNS), microspore enlargements were observed. The medium supplemented with either sodium ferrous ethylene-diamine-tetraacetic acid (NaFeEDTA) or CuSO4·5H2O induced sporophytic cell division in both genotypes. A low frequency of the reprogramming and the presence of non-responsive microspores among the responsive ones in tetrads were found to be related to the viability and exine formation of the microspores. The present study clearly demonstrated that reprogramming occurs much faster in isolated microspore culture than in anther culture. This paves the way for the development of an efficient technique for the production of homozygous lines in cassava. This is the first ever detailed report of microspore reprogramming at the tetrad stage and the first report of microspore embryogenesis induction in cassava with detailed evidence.
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