Evidence map›Paper›PMID 24886790›Full record

ArticlePloS one2014

Immature dengue virus is infectious in human immature dendritic cells via interaction with the receptor molecule DC-SIGN.

Mareike K S Richter, Júlia M da Silva Voorham, Silvia Torres Pedraza, Tabitha E Hoornweg, Denise P I van de Pol, Izabela A Rodenhuis-Zybert, Jan Wilschut, Jolanda M Smit

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 2 pooled it
7.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.

  1. Pooled it
  2. Different Associations betweenInternational journal of environmental research and public health · 2019
    Pooled it
  3. Article
  4. Review
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  11. Animal models for dengue vaccine development and testing.Clinical and experimental vaccine research · 2017
    Review
  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Mareike K S RichterDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Júlia M da Silva VoorhamDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Silvia Torres PedrazaDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands; Grupo Immunovirología, Universidad de Antioquia, Medellín, Colombia.
Tabitha E HoornwegDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Denise P I van de PolDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Izabela A Rodenhuis-ZybertDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Jan WilschutDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Jolanda M SmitDepartment of Medical Microbiology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
University of Groningen · NLUniversity Medical Center Groningen · NLUniversidad de Antioquia · CO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDengue Virus (DENV) is the most common mosquito-borne viral infection worldwide. Important target cells during DENV infection are macrophages, monocytes, and immature dendritic cells (imDCs). DENV-infected cells are known to secrete a large number of partially immature and fully immature particles alongside mature virions. Fully immature DENV particles are considered non-infectious, but antibodies have been shown to rescue their infectious properties. This suggests that immature DENV particles only contribute to the viral load observed in patients with a heterologous DENV re-infection. METHODOLOGY/PRINCIPAL

findingsIn this study, we re-evaluated the infectious properties of fully immature particles in absence and presence of anti-DENV human serum. We show that immature DENV is infectious in cells expressing DC-SIGN. Furthermore, we demonstrate that immature dendritic cells, in contrast to macrophage-like cells, do not support antibody-dependent enhancement of immature DENV. CONCLUSIONS/SIGNIFICANCE: Our data shows that immature DENV can infect imDCs through interaction with DC-SIGN, suggesting that immature and partially immature DENV particles may contribute to dengue pathogenesis during primary infection. Furthermore, since antibodies do not further stimulate DENV infectivity on imDCs we propose that macrophages/monocytes rather than imDCs contribute to the increased viral load observed during severe heterotypic DENV re-infections.

Indexed as

Cell Adhesion MoleculesCells, CulturedDC-Specific ICAM-3 Grabbing NonintegrinDendritic CellsDengue VirusHumansLectins, C-TypeProtein BindingReceptors, Cell SurfaceVirulenceCell Adhesion MoleculesDC-Specific ICAM-3 Grabbing NonintegrinLectins, C-TypeReceptors, Cell Surface

Identifiers

PMID24886790
PMCPMC4041791
OpenAlexW2039821828

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.