ArticleArthritis research & therapy2014
Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage.
Article in Arthritis research & therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
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- Osteocyte-Intrinsic MMP13 exacerbates injury-induced temporomandibular joint osteoarthritis.Osteoarthritis and cartilage · 2026Article
- Metabolomic and proteomic stratification of equine osteoarthritis.Equine veterinary journal · 2025Article
- Proteomics Analysis of Pregnancy in Ewes under Heat Stress Conditions and Melatonin Administration.Animals : an open access journal from MDPI · 2024Article
- Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis.International journal of molecular sciences · 2023Article
- Characterization of the Proteins Secreted by Equine Muscle-Derived Mesenchymal Stem Cells Exposed to Cartilage Explants in Osteoarthritis Model.Stem cell reviews and reports · 2023Article
- Mass spectrometry imaging spatially identifies complex-type N-glycans as putative cartilage degradation markers in human knee osteoarthritis tissue.Analytical and bioanalytical chemistry · 2022Article
- Combining bulk and single-cell RNA-sequencing data to reveal gene expression pattern of chondrocytes in the osteoarthritic knee.Bioengineered · 2021Article
- Article
- Mass Spectrometry Imaging as a Potential Tool to Investigate Human Osteoarthritis at the Tissue Level.International journal of molecular sciences · 2020Review
- From Translation to Protein Degradation as Mechanisms for Regulating Biological Functions: A Review on the SLRP Family in Skeletal Tissues.Biomolecules · 2020Review
- Developing a toolkit for the assessment and monitoring of musculoskeletal ageing.Age and ageing · 2018Review
- Neopeptide Analyser: A software tool for neopeptide discovery in proteomics data.Wellcome open research · 2017Article
- Mass spectrometry imaging: a novel technology in rheumatology.Nature reviews. Rheumatology · 2017Review
- Detecting new microRNAs in human osteoarthritic chondrocytes identifies miR-3085 as a human, chondrocyte-selective, microRNA.Osteoarthritis and cartilage · 2016Article
- Characterization of neopeptides in equine articular cartilage degradation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Article
- Comprehensive protein profiling of synovial fluid in osteoarthritis following protein equalization.Osteoarthritis and cartilage · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
introductionCartilage protein distribution and the changes that occur in cartilage ageing and disease are essential in understanding the process of cartilage ageing and age related diseases such as osteoarthritis. The aim of this study was to investigate the peptide profiles in ageing and osteoarthritic (OA) cartilage sections using matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI).
methodsThe distribution of proteins in young, old and OA equine cartilage was compared following tryptic digestion of cartilage slices and MALDI-MSI undertaken with a MALDI SYNAPT™ HDMS system. Protein identification was undertaken using database searches following multivariate analysis. Peptide intensity differences between young, ageing and OA cartilage were imaged with Biomap software. Analysis of aggrecanase specific cleavage patterns of a crude cartilage proteoglycan extract were used to validate some of the differences in peptide intensity identified. Immunohistochemistry studies validated the differences in protein abundance.
resultsYoung, old and OA equine cartilage was discriminated based on their peptide signature using discriminant analysis. Proteins including aggrecan core protein, fibromodulin, and cartilage oligomeric matrix protein were identified and localised. Fibronectin peptides displayed a stronger intensity in OA cartilage. Age-specific protein markers for collectin-43 and cartilage oligomeric matrix protein were identified. In addition potential fibromodulin and biglycan peptides targeted for degradation in OA were detected.
conclusionsMALDI-MSI provided a novel platform to study cartilage ageing and disease enabling age and disease specific peptides in cartilage to be elucidated and spatially resolved.
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