ArticleEuropean journal of medical research2014
Cryopreservation and replantation of amputated rat hind limbs.
Article in European journal of medical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking.International journal of molecular sciences · 2024Review
- Evaluation of the effects of ectopic replantation on amputate survival in the management of crush traumatic amputations in rats: An experimental study.Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES · 2024Article
- Significantly Improved Cold Preservation of Rat Hind Limb Vascularized Composite Allografts Using the New PrC-210 Free Radical Scavenger.International journal of molecular sciences · 2024Article
- Digit Replantation Practice: A Comparison of Three Rat Models.Journal of hand and microsurgery · 2023Article
- Article
- Cryopreservation of tissues and organs: present, bottlenecks, and future.Frontiers in veterinary science · 2023Review
- Thermal Analyses of Nanowarming-Assisted Recovery of the Heart From Cryopreservation by Vitrification.Journal of heat transfer · 2022Article
- Preoperative Cryopreservation Promotes Digital Survival after Digit Replantation.Computational and mathematical methods in medicine · 2022Article
- Analysis of crystallization during rewarming in suboptimal vitrification conditions: a semi-empirical approach.Cryobiology · 2021Article
- Perfusion, cryopreservation, and nanowarming of whole hearts using colloidally stable magnetic cryopreservation agent solutions.Science advances · 2021Article
- Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells.Cellular and molecular neurobiology · 2020Article
- Thermal analysis of marginal conditions to facilitate cryopreservation by vitrification using a semi-empirical approach.Cryobiology · 2019Article
- Genetic suppression of cryoprotectant toxicity.Cryobiology · 2019Article
- Various changes in cryopreserved acellular nerve allografts at -80°C.Neural regeneration research · 2018Article
- Social, economic, and policy implications of organ preservation advances.Current opinion in organ transplantation · 2018Review
- Thermal Analyses of a Human Kidney and a Rabbit Kidney During Cryopreservation by Vitrification.Journal of biomechanical engineering · 2018Article
- Tissue conservation for transplantation.Innovative surgical sciences · 2017Review
- The promise of organ and tissue preservation to transform medicine.Nature biotechnology · 2017Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn spite of the relatively high success rate of limb replantation, many patients cannot undergo replantation surgery because the preservation time of an amputated limb is only about six hours. In addition, although allotransplantation of composite tissues is being performed more commonly with increasingly greater success rates, the shortage of donors limits the number of patients that can be treated. So the purpose of this study is to examine the feasibility of cryopreservation and replantation of limbs in a rat model.
methodsTwelve five-month-old Sprague-Dawley rats were divided evenly into group A (above-knee amputation) and group B (Syme's amputation). One hind limb was amputated from each rat. The limbs were irrigated with cryoprotectant, cooled in a controlled manner to -140°C, and placed in liquid nitrogen. Thawing and replantation were performed 14 days later.
resultsIn group A, the limbs became swollen after restoration of blood flow resulting in blood vessel compression and all replantations failed. In group B, restoration of blood flow was noted in all limbs after replantation. In one case, the rat chewed the replanted limb and replantation failed. The other five rats were followed for three months with no abnormalities noted in the replanted limbs.
conclusionsLimbs with a minimal amount of muscle tissue can be successfully cryopreserved and replanted.
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