ArticleBMC cancer2014
Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells.
Article in BMC cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- DNA Methylation Biomarker Discovery for Colorectal Cancer Diagnosis Assistance Through Integrated Analysis.Cancer informatics · 2025Article
- Epigenome-Wide DNA Methylation Profiling in Colorectal Cancer and Normal Adjacent Colon Using Infinium Human Methylation 450K.Diagnostics (Basel, Switzerland) · 2022Article
- Four methylation-driven genes detected by linear discriminant analysis model from early-stage colorectal cancer and their methylation levels in cell-free DNA.Frontiers in oncology · 2022Article
- Article
- Transcriptome Analysis Identifies Novel Mechanisms Associated with the Antitumor Effect of Chitosan-Stabilized Selenium Nanoparticles.Pharmaceutics · 2021Article
- Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model.Scientific reports · 2021Article
- ADHFE1 is a correlative factor of patient survival in cancer.Open life sciences · 2021Article
- The incidence and risk factors of sessile serrated adenomas in left side colon cancer patients after curative surgery.Medicine · 2020Observational
- Genome-wide DNA methylation profiles of low- and high-grade adenoma reveals potential biomarkers for early detection of colorectal carcinoma.Clinical epigenetics · 2020Article
- Modeling the effect of prolonged ethanol exposure on global gene expression and chromatin accessibility in normal 3D colon organoids.PloS one · 2020Article
- Hypermethylation Of ADHFE1 Promotes The Proliferation Of Colorectal Cancer Cell Via Modulating Cell Cycle Progression.OncoTargets and therapy · 2019Article
- Review
- Identification of Hyper-Methylated Tumor Suppressor Genes-Based Diagnostic Panel for Esophageal Squamous Cell Carcinoma (ESCC) in a Chinese Han Population.Frontiers in genetics · 2018Article
- Genome-wide DNA methylation profiling reveals novel epigenetic signatures in squamous cell lung cancer.BMC genomics · 2017Article
- Identification of regulatory role of DNA methylation in colon cancer gene expression via systematic bioinformatics analysis.Medicine · 2017Article
- Article
- Alcohol Injury Damages Intestinal Stem Cells.Alcoholism, clinical and experimental research · 2017Article
- Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma.World journal of gastroenterology · 2016Article
- Reconstruction of Tissue-Specific Metabolic Networks Using CORDA.PLoS computational biology · 2016Article
- The association, clinicopathological significance, and diagnostic value ofOncoTargets and therapy · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe hypermethylation of Alcohol dehydrogenase iron containing 1 (ADHFE1) was recently reported to be associated with colorectal cancer (CRC) differentiation. However, the effect of alcohol on ADHFE1 hypermethylation in CRC is still unclear.
methodsThe methylation status and expression levels of ADHFE1 were investigated in primary tumor tissues and adjacent normal tissues of 73 patients with CRC, one normal colon cell line, and 4 CRC cell lines (HT-29, SW480, DLD-1, and LoVo) by quantitative methylation-specific polymerase chain reaction (QMSP) and real-time reverse transcription polymerase chain reaction (real time PCR), respectively. The effect of alcohol on the methylation status of ADHFE1 was analyzed in HT-29, SW480, DLD-1, and CCD18Co cells using QMSP, real-time PCR, immunoblot, and cell proliferation assay.
resultsADHFE1 was hypermethylated in 69 of 73 CRC tissues (95%) compared to adjacent normal tissues (p<0.05). The mRNA expression of ADHFE1 was significantly reduced in CRC compared to adjacent normal tissues (p<0.05) and its expression was decreased in the alcohol consumption group (p<0.05). ADHFE1 was hypermethylated and its expression was decreased in 4 CRC cell lines compared with normal colon cell line. Alcohol induced hypermethylation of ADHFE1, decreased its expression, and stimulated cell proliferation of HT-29, SW480, and DLD-1cells.
conclusionThese results demonstrate that the promoter hypermethylation of ADHFE1 is frequently present in CRC and alcohol induces methylation-mediated down expression of ADHFE1 and proliferation of CRC cells.
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