ArticleBMC genomics2014
Transcriptome assemblies for studying sex-biased gene expression in the guppy, Poecilia reticulata.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 94 citations in OpenAlex.
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- Identification of a Candidate Sex Determination Region and Sex-specific Molecular Markers of Spinibarbus caldwelli.Marine biotechnology (New York, N.Y.) · 2026Article
- Mitogenomic Characterization and Phylogenetic Insights of the Ornamental Sail-Fin Molly (Poecilia velifera) in Non-Native Indonesian Waters.Biochemical genetics · 2026Article
- Insights into Human Ciliopathies: Gene Silencing ofmicroPublication biology · 2026Article
- Identification of the male-specific region on the guppy Y Chromosome from a haplotype-resolved assembly.Genome research · 2025Article
- Disentangling genetic, plastic and social learning drivers of sex-specific foraging behaviour in Trinidadian guppies (Proceedings. Biological sciences · 2024Article
- The Genetic Basis Underpinning Sexually Selected Traits across Different Animal Lineages: Are There Genetic Mechanisms in Common?Animals : an open access journal from MDPI · 2024Review
- Sex-biased gene expression at single-cell resolution: cause and consequence of sexual dimorphism.Evolution letters · 2023Article
- Experimental Life History Evolution Results in Sex-specific Evolution of Gene Expression in Seed Beetles.Genome biology and evolution · 2023Article
- Sex differences in gene expression and alternative splicing in the Chinese horseshoe bat.PeerJ · 2023Article
- Sex differences in immune gene expression in the brain of a small shorebird.Immunogenetics · 2022Article
- Developmental RNA-Seq transcriptomics of haploid germ cells and spermatozoa uncovers novel pathways associated with teleost spermiogenesis.Scientific reports · 2022Article
- Invasion and rapid adaptation of guppies (Evolutionary applications · 2021Article
- Article
- Patterns of recent natural selection on genetic loci associated with sexually differentiated human body size and shape phenotypes.PLoS genetics · 2021Article
- Article
- Genomic stability through time despite decades of exploitation in cod on both sides of the Atlantic.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Divergence and Remarkable Diversity of the Y Chromosome in Guppies.Molecular biology and evolution · 2021Article
- Transkingdom network analysis provides insight into host-microbiome interactions in Atlantic salmon.Computational and structural biotechnology journal · 2021Article
- Improved Reference Genome Uncovers Novel Sex-Linked Regions in the Guppy (Poecilia reticulata).Genome biology and evolution · 2020Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSexually dimorphic phenotypes are generally associated with differential gene expression between the sexes. The study of molecular evolution and genomic location of these differentially expressed, or sex-biased, genes is important for understanding inter-sexual divergence under sex-specific selection pressures. Teleost fish provide a unique opportunity to examine this divergence in the presence of variable sex-determination mechanisms of recent origin. The guppy, Poecilia reticulata, displays sexual dimorphism in size, ornaments, and behavior, traits shaped by natural and sexual selection in the wild.
resultsTo gain insight into molecular mechanisms underlying the guppy's sexual dimorphism, we assembled a reference transcriptome combining genome-independent as well as genome-guided assemblies and analyzed sex-biased gene expression between different tissues of adult male and female guppies. We found tissue-associated sex-biased expression of genes related to pigmentation, signal transduction, and spermatogenesis in males; and growth, cell-division, extra-cellular matrix organization, nutrient transport, and folliculogenesis in females. While most sex-biased genes were randomly distributed across linkage groups, we observed accumulation of ovary-biased genes on the sex linkage group, LG12. Both testis-biased and ovary-biased genes showed a significantly higher rate of non-synonymous to synonymous substitutions (dN/dS) compared to unbiased genes. However, in somatic tissues only female-biased genes, including those co-expressed in multiple tissues, showed elevated ratios of non-synonymous substitutions.
conclusionsOur work identifies a set of annotated gene products that are candidate factors affecting sexual dimorphism in guppies. The differential genomic distribution of gonad-biased genes provides evidence for sex-specific selection pressures acting on the nascent sex chromosomes of the guppy. The elevated rates of evolution of testis-biased and female-biased genes indicate differing evolution under distinct selection pressures on the reproductive versus non-reproductive tissues.
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