ArticleRadiation oncology (London, England)2014
Integrating microRNA and mRNA expression profiles in response to radiation-induced injury in rat lung.
Article in Radiation oncology (London, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 35 citations in OpenAlex.
- Enhancing radioprotection: exploring the impact of L-carnitine supplementation on the oxidative stress in the liver.Molecular biology reports · 2024Article
- Tetrahydrobiopterin metabolism attenuates ROS generation and radiosensitivity through LDHA S-nitrosylation: novel insight into radiogenic lung injury.Experimental & molecular medicine · 2024Article
- Effects of RadioactiveInternational journal of molecular sciences · 2023Article
- METTL3 Mediates Epithelial-Mesenchymal Transition by Modulating FOXO1 mRNA NAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Effects of Internal Exposure of RadioactiveCurrent issues in molecular biology · 2023Article
- Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.Respiratory research · 2022Article
- Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug.International journal of molecular sciences · 2021Article
- Biological impacts on the lungs in rats internally exposed to radioactiveScientific reports · 2021Article
- The Crosstalk of Adipose-Derived Stem Cells (ADSC), Oxidative Stress, and Inflammation in Protective and Adaptive Responses.International journal of molecular sciences · 2020Review
- Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway.Clinical interventions in aging · 2020Article
- Transcriptome alteration spectrum in rat lung induced by radiotherapy.Scientific reports · 2019Article
- Radiation-Induced Normal Tissue Damage: Oxidative Stress and Epigenetic Mechanisms.Oxidative medicine and cellular longevity · 2019Review
- MicroRNA dysregulation in lung injury: the role of the miR-26a/EphA2 axis in regulation of endothelial permeability.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- MicroRNAs in mucosal inflammation.Journal of molecular medicine (Berlin, Germany) · 2017Review
- Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats.Scientific reports · 2017Article
- Emergence of miR-34a in radiation therapy.Critical reviews in oncology/hematology · 2017Review
- Mechanisms of the alternative activation of macrophages and non-coding RNAs in the development of radiation-induced lung fibrosis.World journal of biological chemistry · 2016Review
- Identification of hub genes of pneumocyte senescence induced by thoracic irradiation using weighted gene co‑expression network analysis.Molecular medicine reports · 2016Article
- Radiation-induced pulmonary injury accelerated pulmonary metastasis in a mouse model of breast cancer.Oncology letters · 2015Article
- Integrative analysis of gene expression in response to low-dose ionizing radiation in a human skin model.Medical oncology (Northwood, London, England) · 2015Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeExposure to radiation provokes cellular responses, which are likely regulated by gene expression networks. MicroRNAs are small non-coding RNAs, which regulate gene expression by promoting mRNA degradation or inhibiting protein translation. The expression patterns of both mRNA and miRNA during the radiation-induced lung injury (RILI) remain less characterized and the role of miRNAs in the regulation of this process has not been studied. The present study sought to evaluate miRNA and mRNA expression profiles in the rat lung after irradiation. METHODS AND MATERIALS: Male Wistar rats were subjected to single dose irradiation with 20 Gy using 6 MV x-rays to the right lung. (A dose rate of 5 Gy/min was applied). Rats were sacrificed at 3, 12 and 26 weeks after irradiation, and morphological changes in the lung were examined by haematoxylin and eosin. The miRNA and mRNA expression profiles were evaluated by microarrays and followed by quantitative RT-PCR analysis.
resultsA cDNA microarray analysis found 2183 transcripts being up-regulated and 2917 transcripts down-regulated (P ≤ 0.05, ≥2.0 fold change) in the lung tissues after irradiation. Likewise, a miRNAs microarray analysis indicated 15 miRNA species being up-regulated and 8 down-regulated (P ≤ 0.05). Subsequent bioinformatics analyses of the differentially expressed mRNA and miRNAs revealed that alterations in mRNA expression following irradiation were negatively correlated with miRNAs expression.
conclusionsOur results provide evidence indicating that irradiation induces alterations of mRNA and miRNA expression in rat lung and that there is a negative correlation of mRNA and miRNA expression levels after irradiation. These findings significantly advance our understanding of the regulatory mechanisms underlying the pathophysiology of radiation-induced lung injury. In summary, RILI does not develop gradually in a linear process. In fact, different cell types interact via cytokines in a very complex network. Furthermore, this study suggests that microRNAs may serve an important role in the pathogenesis of RILI and that understanding their role in RILI may have a significant effect on patient management and diagnosis in the future.
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