ArticleMicrobial cell factories2014
Engineering Escherichia coli K12 MG1655 to use starch.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 24 citations in OpenAlex.
- Diversity and Bioprospection of Gram-positive Bacteria Derived from a Mayan Sinkhole.Microbial ecology · 2024Article
- [Laboratory study on bacteriological aspects of beverages and thickeners for dysphagia patients : Growth survey of Escherichia coli on thickeners and beverages].Zeitschrift fur Gerontologie und Geriatrie · 2022Article
- Inducible directed evolution of complex phenotypes in bacteria.Nucleic acids research · 2022Article
- Molecular characterization of Histomonas meleagridis exoproteome with emphasis on protease secretion and parasite-bacteria interaction.PloS one · 2019Article
- Antibacterial Metal Oxide Nanoparticles: Challenges in Interpreting the Literature.Current pharmaceutical design · 2018Review
- Analysis on evolutionary relationship of amylases from archaea, bacteria and eukaryota.World journal of microbiology & biotechnology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo attain a sustainable bioeconomy, fuel, or valuable product, production must use biomass as substrate. Starch is one of the most abundant biomass resources and is present as waste or as a food and agroindustry by-product. Unfortunately, Escherichia coli, one of the most widely used microorganisms in biotechnological processes, cannot use starch as a carbon source.
resultsWe engineered an E. coli strain capable of using starch as a substrate. The genetic design employed the native capability of the bacterium to use maltodextrins as a carbon source plus expression and secretion of its endogenous α-amylase, AmyA, in an adapted background. Biomass production improved using 35% dissolved oxygen and pH 7.2 in a controlled bioreactor.
conclusionThe engineered E. coli strain can use starch from the milieu and open the possibility of optimize the process to use agroindustrial wastes to produce biofuels and other valuable chemicals.
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