ArticleBMC genomics2014
Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- MicroRNAs as diagnostic and prognostic biomarkers for viral myocarditis: A systematic review and meta-analysis.International journal of cardiology. Heart & vasculature · 2026Article
- Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and cats.Veterinary clinical pathology · 2025Review
- Correlation between circulating microRNAs and vascular biomarkers in type 2 diabetes based upon physical activity: a biochemical analytic study.BMC endocrine disorders · 2025Observational
- Widely conserved miRNAs in buffalo milk extracellular vesicles survive gastrointestinal digestion and potentially target neural and immunomodulatory contexts.Frontiers in nutrition · 2025Article
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- Circulating microRNAs as Reliable Tumor Biomarkers: Opportunities and Challenges Facing Clinical Application.The Journal of pharmacology and experimental therapeutics · 2023Review
- Circulating MicroRNA Panel as a Diagnostic Marker for Hepatocellular Carcinoma.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2022Article
- Gestational Diabetes is Associated with an Increased Expression of miR-27a in Peripheral Blood Mononuclear Cells.Molecular diagnosis & therapy · 2022Article
- Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR.Biomedicines · 2022Article
- Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids.BMC biotechnology · 2021Article
- Evaluation of five column-based isolation kits and their ability to extract miRNA from human milk.Journal of cellular and molecular medicine · 2021Article
- Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods.Diagnostics (Basel, Switzerland) · 2021Review
- Measurements Methods for the Development of MicroRNA-Based Tests for Cancer Diagnosis.International journal of molecular sciences · 2021Review
- Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Challenges in Using Circulating Micro-RNAs as Biomarkers for Cardiovascular Diseases.International journal of molecular sciences · 2020Review
- An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells.Frontiers in immunology · 2020Article
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- A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification.Lab on a chip · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmicroRNAs (miRNAs) play crucial roles in major biological processes and their deregulations are often associated with human malignancies. As such, they represent appealing candidates as targets of innovative therapies. Another interesting aspect of their biology is that they are present in various biological fluids where, advantageously, they appear to be very stable. A plethora of studies have now reported their potential as biomarkers that can be used in diagnosis, prognosis and/or theranostic issues. However, the application of circulating miRNAs in clinical practices still requires the identification of highly efficient, robust and reproducible methods for their isolation from biological samples.In that context, we performed an independent cross-comparison of three commercially available RNA extraction kits for miRNAs isolation from human blood samples (Qiagen and Norgen kits as well as the new NucleoSpin miRNAs Plasma kit from Macherey-Nagel). miRNAs were further profiled using the Taqman Low Density Array technology.
resultsWe found that, although these 3 kits had equal performances in extracting miRNAs from peripheral blood mononuclear cells, the Macherey-Nagel kit presented several advantages when isolating miRNAs from sera. Besides, our results have indicated that, depending on the quantity of the biological samples used, the extraction procedure directly impacted on the G/C composition of the miRNAs detected.
conclusionOverall, our study contributes to the definition of a reliable framework for profiling circulating miRNAs.
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