ArticleBMC biology2014
Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Predicted functional consequences of WNT ligand mutations in colorectal cancer.Biophysical journal · 2025Article
- Mechanistic insights into Wnt-β-catenin pathway activation and signal transduction.Nature reviews. Molecular cell biology · 2025Review
- Synthesis and Frizzled-receptor binding of a WNT5A hairpin-3 peptide.Chemical communications (Cambridge, England) · 2024Article
- CircMCTP2 enhances the progression of bladder cancer by regulating the miR-99a-5p/FZD8 axis.Journal of the Egyptian National Cancer Institute · 2024Article
- The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra.BMC biology · 2021Article
- Article
- Novel Small Molecules against Two Binding Sites of Wnt2 Protein as potential Drug Candidates for Colorectal Cancer: A Structure Based Virtual Screening Approach.Iranian journal of pharmaceutical research : IJPR · 2020Article
- Fatty acid recognition in the Frizzled receptor family.The Journal of biological chemistry · 2019Review
- Structural and dynamic characterization of human Wnt2-Fzd7 complex using computational approaches.Journal of molecular modeling · 2018Article
- From top to bottom: Cell polarity in Hedgehog and Wnt trafficking.BMC biology · 2018Review
- The function of endocytosis in Wnt signaling.Cellular and molecular life sciences : CMLS · 2018Review
- ERAD-dependent control of the Wnt secretory factor Evi.The EMBO journal · 2018Article
- The crystal structure of full-length Sizzled fromThe Journal of biological chemistry · 2017Article
- Characterization of secondary structure and lipid binding behavior of N-terminal saposin like subdomain of human Wnt3a.Archives of biochemistry and biophysics · 2017Article
- Article
- De novo HAPLN1 expression hallmarks Wnt-induced stem cell and fibrogenic networks leading to aggressive human hepatocellular carcinomas.Oncotarget · 2016Article
- Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation.The Journal of biological chemistry · 2016Article
- Mutations in DDX3X Are a Common Cause of Unexplained Intellectual Disability with Gender-Specific Effects on Wnt Signaling.American journal of human genetics · 2015Article
- Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.The Journal of investigative dermatology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundWnt proteins are a family of secreted signaling molecules that regulate key developmental processes in metazoans. The molecular basis of Wnt binding to Frizzled and LRP5/6 co-receptors has long been unknown due to the lack of structural data on Wnt ligands. Only recently, the crystal structure of the Wnt8-Frizzled8-cysteine-rich-domain (CRD) complex was solved, but the significance of interaction sites that influence Wnt signaling has not been assessed.
resultsHere, we present an extensive structure-function analysis of mouse Wnt3a in vitro and in vivo. We provide evidence for the essential role of serine 209, glycine 210 (site 1) and tryptophan 333 (site 2) in Fz binding. Importantly, we discovered that valine 337 in the site 2 binding loop is critical for signaling without contributing to binding. Mutations in the presumptive second CRD binding site (site 3) partly abolished Wnt binding. Intriguingly, most site 3 mutations increased Wnt signaling, probably by inhibiting Wnt-CRD oligomerization. In accordance, increasing amounts of soluble Frizzled8-CRD protein modulated Wnt3a signaling in a biphasic manner.
conclusionsWe propose a concentration-dependent switch in Wnt-CRD complex formation from an inactive aggregation state to an activated high mobility state as a possible modulatory mechanism in Wnt signaling gradients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.