ArticleBMC research notes2014
A method for generating highly multiplexed ChIP-seq libraries.
Article in BMC research notes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Repurposing the lineage-determining transcription factor Atoh1 without redistributing its genomic binding sites.Frontiers in cell and developmental biology · 2022Article
- Effects of Hst3p inhibition inFrontiers in cellular and infection microbiology · 2022Article
- Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.EMBO reports · 2021Article
- Identification of a dynamic gene regulatory network required for pluripotency factor-induced reprogramming of mouse fibroblasts and hepatocytes.The EMBO journal · 2021Article
- The lysine-specific methyltransferase KMT2C/MLL3 regulates DNA repair components in cancer.EMBO reports · 2019Article
- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.Methods in molecular biology (Clifton, N.J.) · 2019Article
- RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss.Cell reports · 2018Article
- Tripartite Chromatin Localization of Budding Yeast Shugoshin Involves Higher-Ordered Architecture of Mitotic Chromosomes.G3 (Bethesda, Md.) · 2018Article
- The Histone Variant MacroH2A Blocks Cellular Reprogramming by Inhibiting Mesenchymal-to-Epithelial Transition.Molecular and cellular biology · 2018Article
- The dual role of LSD1 and HDAC3 in STAT5-dependent transcription is determined by protein interactions, binding affinities, motifs and genomic positions.Nucleic acids research · 2017Article
- Genome-wide studies reveal novel and distinct biological pathways regulated by SIN3 isoforms.BMC genomics · 2016Article
- Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.Nature structural & molecular biology · 2016Article
- Genome-wide errant targeting by Hairy.eLife · 2015Article
- Ets homologous factor regulates pathways controlling response to injury in airway epithelial cells.Nucleic acids research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe barcoding of next generation sequencing libraries has become an essential part of the experimental design. Barcoding not only allows the sequencing of more than one sample per lane, but also reduces technical bias. However, current barcoding strategies impose significant limitations and/or technical barriers in their implementation for ChIP-sequencing.
findingsConverting Y-shaped sequencing adapters to double stranded DNA prior to agarose gel size selection reduces adapter dimer contamination and quantitating the number of cycles required for amplification of the library with qPCR prior to library amplification eliminates library over-amplification.
conclusionsWe describe an efficient and cost effective method for making barcoded ChIP-seq libraries for sequencing on the Illumina platform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.