ArticleParasites & vectors2014
Laboratory assessment of sensitive molecular tools for detection of low levels of Echinococcus multilocularis-eggs in fox (Vulpes vulpes) faeces.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Annual assessment ofEFSA journal. European Food Safety Authority · 2026Article
- Systemic remodeling of the multi-organ virome following Echinococcus infection in mice.BMC microbiology · 2026Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2025Article
- Prevalence and genetic diversity of the lung nematode Eucoleus aerophilus in red foxes (Vulpes vulpes) in Central Europe (Poland) assessed by PCR and flotation.Scientific reports · 2024Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2024Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2023Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2022Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2021Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2021Article
- Comparison of Two DNA Extraction Methods and Two PCRs for Detection ofAnimals : an open access journal from MDPI · 2020Article
- Species Detection within thePathogens (Basel, Switzerland) · 2020Article
- Annual assessment ofEFSA journal. European Food Safety Authority · 2019Article
- Validation of PCR-based protocols for the detection ofFood and waterborne parasitology · 2019Article
- Echinococcosis: Advances in the 21st Century.Clinical microbiology reviews · 2019Review
- Annual assessment ofEFSA journal. European Food Safety Authority · 2018Article
- Assessment ofEFSA journal. European Food Safety Authority · 2017Article
- Assessment ofEFSA journal. European Food Safety Authority · 2016Article
- Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard.Infection ecology & epidemiology · 2016Article
- A semi-automated magnetic capture probe based DNA extraction and real-time PCR method applied in the Swedish surveillance of Echinococcus multilocularis in red fox (Vulpes vulpes) faecal samples.Parasites & vectors · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn endemic areas with very low infection prevalence, the frequency and intensity of Echinococcus multilocularis can be extremely low. This necessitates efficient, specific and sensitive molecular tools. We wanted to compare the existing molecular tools, used in the Norwegian national surveillance programme, and compare these with new techniques for detection of this zoonotic pathogen in fox faeces. Here we present the results of screening samples containing a known level of E. multilocularis eggs with two highly sensitive DNA isolation and extraction methods combined with one conventional PCR and three real-time PCR methods for detection.
methodsWe performed a comparison of two extraction protocols; one based on sieving of faecal material and one using targeted DNA sampling. Four methods of molecular detection were tested on E. multilocularis-egg spiked fox faeces.
resultsThere were significant differences between the multiplex PCR/egg sieving DNA extraction methods compared to the new DNA fishing method and the three real-time PCR assays. Results also indicate that replicates of the PCR-reactions improve detection sensitivity when egg numbers are low.
conclusionsThe results indicate that the use of real-time PCR combined with targeted DNA extraction, improves the sensitivity of E. multilocularis detection in faecal samples containing low numbers of E. multilocularis eggs. Results also indicate the importance of replicates of the PCR-reactions when pathogen levels are low.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.