ArticleBMC infectious diseases2014
Performance of seven serological assays for diagnosing tularemia.
Article in BMC infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Immunosensors and Immunoassays to DetectBiosensors · 2026Review
- Not Tuberculosis After All: The "Hare-Raising Case" of Tularemia in a Patient With Advanced HIV Infection.Case reports in infectious diseases · 2026Article
- First molecular detection ofVeterinary and animal science · 2025Article
- Seroepidemiology of Human Tularemia-Systematic Review and Meta-analysis of Seroprevalence Studies.Open forum infectious diseases · 2024Article
- Article
- Development of a novel sandwich immunoassay based on targeting recombinant Francisella outer membrane protein A for the diagnosis of tularemia.Frontiers in cellular and infection microbiology · 2024Article
- Rapid Detection of Lipopolysaccharide and Whole Cells ofMicromachines · 2022Article
- Seroepidemiology, Spatial Distribution, and Risk Factors ofThe American journal of tropical medicine and hygiene · 2020Article
- Rapid and Culture Free Identification ofFrontiers in microbiology · 2020Article
- Review
- Article
- Epidemiological survey of tularemia in Ilam Province, west of Iran.BMC infectious diseases · 2019Article
- [Painful lymphadenopathy after an insect bite-a case report].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2019Article
- Usefulness of a single-assay chemiluminescence test (Tularaemia VIRCLIA IgG + IgM monotest) for the diagnosis of human tularemia. Comparison of five serological tests.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Immunoglobulin G subclass switching impacts sensitivity of an immunoassay targeting Francisella tularensis lipopolysaccharide.PloS one · 2018Article
- Tularemia in Germany-A Re-emerging Zoonosis.Frontiers in cellular and infection microbiology · 2018Review
- Evaluation of In-House and Commercial Serological Tests for Diagnosis of Human Tularemia.Journal of clinical microbiology · 2018Article
- Rapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques.The open microbiology journal · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTularemia is a rare zoonotic disease caused by the Gram-negative bacterium Francisella tularensis. Serology is frequently the preferred diagnostic approach, because the pathogen is highly infectious and difficult to cultivate. The aim of this retrospective study was to determine the diagnostic accuracy of tularemia specific tests.
methodsThe Serazym®Anti-Francisella tularensis ELISA, Serion ELISA classic Francisella tularensis IgG/IgM, an in-house ELISA, the VIRapid® Tularemia immunochromatographic test, an in-house antigen microarray, and a Western Blot (WB) assay were evaluated. The diagnosis tularemia was established using a standard micro-agglutination assay. In total, 135 sera from a series of 110 consecutive tularemia patients were tested.
resultsThe diagnostic sensitivity and diagnostic specificity of the tests were VIRapid (97.0% and 84.0%), Serion IgG (96.3% and 96.8%), Serion IgM (94.8% and 96.8%), Serazym (97.0% and 91.5%), in-house ELISA (95.6% and 76.6%), WB (93.3% and 83.0%), microarray (91.1% and 97.9%).
conclusionsThe diagnostic value of the commercial assays was proven, because the diagnostic accuracy was >90%. The diagnostic sensitivity of the in-house ELISA and the WB were acceptable, but the diagnostic accuracy was <90%. Interestingly, the antigen microarray test was very specific and had a very good positive predictive value.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.