ArticleBMC genomics2014
Genome analysis and CRISPR typing of Salmonella enterica serovar Virchow.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- CRISPR Typing of Clinical Strains ofIranian journal of public health · 2023Article
- Development and Evaluation of Alternative Methods to Identify the Three Most Common Serotypes ofMicroorganisms · 2021Article
- Shining light on a deep-sea bacterial symbiont population structure with CRISPR.Microbial genomics · 2021Article
- Genomic Identification of Multidrug-ResistantPathogens (Basel, Switzerland) · 2021Article
- Comparative Genomic Analyses and CRISPR-Cas Characterization ofFrontiers in microbiology · 2021Article
- Iron-Uptake Systems of Chicken-AssociatedMicroorganisms · 2020Review
- Prophages in Salmonella enterica: a driving force in reshaping the genome and physiology of their bacterial host?Molecular microbiology · 2019Review
- Review
- Exploiting CRISPR-Cas to manipulateeLife · 2017Article
- Characterization and Exploitation of CRISPR Loci inFrontiers in microbiology · 2017Article
- Metabolic characteristics of dominant microbes and key rare species from an acidic hot spring in Taiwan revealed by metagenomics.BMC genomics · 2015Article
- Knowledge-based discovery for designing CRISPR-CAS systems against invading mobilomes in thermophiles.Systems and synthetic biology · 2015Article
- Comparative Analysis of the Orphan CRISPR2 Locus in 242 Enterococcus faecalis Strains.PloS one · 2015Article
- Diversity and antimicrobial resistance of Salmonella enterica isolates from surface water in Southeastern United States.Applied and environmental microbiology · 2014Article
- Salmonella Virchow Infection of the Chicken Elicits Cellular and Humoral Systemic and Mucosal Responses, but Limited Protection to Homologous or Heterologous Re-Challenge.Frontiers in veterinary science · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella enterica subsp. enterica serovar Virchow has been recognized as a significant health burden in Asia, Australia and Europe. In addition to its global distribution, S. Virchow is clinically significant due to the frequency at which it causes invasive infections and its association with outbreaks arising from food-borne transmission. Here, we examine the genome of an invasive isolate of S. Virchow SVQ1 (phage type 8) from an outbreak in southeast Queensland, Australia. In addition to identifying new potential genotyping targets that could be used for discriminating between S. Virchow strains in outbreak scenarios, we also aimed to carry out a comprehensive comparative analysis of the S. Virchow genomes.
resultsGenome comparisons between S. Virchow SVQ1 and S. Virchow SL491, a previously published strain, identified a high degree of genomic similarity between the two strains with fewer than 200 single nucleotide differences. Clustered Regularly Interspaced Palindromic Repeats (CRISPR) regions were identified as a highly variable region that could be used to discriminate between S. Virchow isolates. We amplified and sequenced the CRISPR regions of fifteen S. Virchow isolates collected from seven different outbreaks across Australia. We observed three allelic types of the CRISPR region from these isolates based on the presence/absence of the spacers and were able to discriminate S. Virchow phage type 8 isolates originating from different outbreaks. A comparison with 27 published Salmonella genomes found that the S. Virchow SVQ1 genome encodes 11 previously described Salmonella Pathogenicity Islands (SPI), as well as additional genomic islands including a remnant integrative conjugative element that is distinct from SPI-7. In addition, the S. Virchow genome possesses a novel prophage that encodes the Type III secretion system effector protein SopE, a key Salmonella virulence factor. The prophage shares very little similarity to the SopE prophages found in other Salmonella serovars suggesting an independent acquisition of sopE.
conclusionsThe availability of this genome will serve as a genome template and facilitate further studies on understanding the virulence and global distribution of the S. Virchow serovar, as well as the development of genotyping methods for outbreak investigations.
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