ArticleMicrobial cell factories2014
Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Fine modulation of carbon flow in central carbon metabolism via ribosome-binding site modification inMetabolic engineering communications · 2026Article
- Enhanced production of l-fuculose by Escherichia coli engineered via genome-scale metabolic modeling.PloS one · 2026Article
- Identification of a novel NADPH generation reaction in the pentose phosphate pathway inJournal of bacteriology · 2024Article
- Loss of function of Hog1 improves glycerol assimilation in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2023Article
- Advances in the optimization of central carbon metabolism in metabolic engineering.Microbial cell factories · 2023Review
- Construction of Multiscale Genome-Scale Metabolic Models: Frameworks and Challenges.Biomolecules · 2022Review
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- Intensifying niacin-based biosynthesis of NADBiotechnology letters · 2021Article
- Production of 3-Hydroxypropanoic Acid From Glycerol by Metabolically Engineered Bacteria.Frontiers in bioengineering and biotechnology · 2019Review
- Engineering an aldehyde dehydrogenase toward its substrates, 3-hydroxypropanal and NADScientific reports · 2017Article
- Investigation of the key factors on 3-hydroxypropionic acid production with different recombinant strains.3 Biotech · 2017Article
- Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli.Microbial cell factories · 2017Article
- Metabolic anchor reactions for robust biorefining.Metabolic engineering · 2017Article
- Exploring Lactobacillus reuteri DSM20016 as a biocatalyst for transformation of longer chain 1,2-diols: Limits with microcompartment.PloS one · 2017Article
- Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.Microbial cell factories · 2016Article
- Furan, phenolic, and heptelidic acid derivatives produced byFood science and biotechnology · 2016Article
- Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol.Biotechnology for biofuels · 2016Review
- Co-evolution of strain design methods based on flux balance and elementary mode analysis.Metabolic engineering communications · 2015Review
- Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch.Microbial cell factories · 2015Article
- Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum.Biotechnology for biofuels · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background3-hydroxypropionic acid (3HP) is an important chemical precursor for the production of bioplastics. Microbial production of 3HP from glycerol has previously been developed through the optimization of culture conditions and the 3HP biosynthesis pathway. In this study, a novel strategy for improving 3HP production in Escherichia coli was investigated by the modification of central metabolism based on a genome-scale metabolic model and experimental validation.
resultsMetabolic simulation identified the double knockout of tpiA and zwf as a candidate for improving 3HP production. A 3HP-producing strain was constructed by the expression of glycerol dehydratase and aldehyde dehydrogenase. The double knockout of tpiA and zwf increased the percentage carbon-molar yield (C-mol%) of 3HP on consumed glycerol 4.4-fold (20.1 ± 9.2 C-mol%), compared to the parental strain. Increased extracellular methylglyoxal concentrations in the ΔtpiA Δzwf strain indicated that glycerol catabolism was occurring through the methylglyoxal pathway, which converts dihydroxyacetone phosphate to pyruvate, as predicted by the metabolic model. Since the ΔtpiA Δzwf strain produced abundant 1,3-propanediol as a major byproduct (37.7 ± 13.2 C-mol%), yqhD, which encodes an enzyme involved in the production of 1,3-propanediol, was disrupted in the ΔtpiA Δzwf strain. The 3HP yield of the ΔtpiA Δzwf ΔyqhD strain (33.9 ± 1.2 C-mol%) was increased 1.7-fold further compared to the ΔtpiA Δzwf strain and by 7.4-fold compared to the parental strain.
conclusionThis study successfully increased 3HP production by 7.4-fold in the ΔtpiA Δzwf ΔyqhD E. coli strain by the modification of the central metabolism, based on metabolic simulation and experimental validation of engineered strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.