ArticleBMC microbiology2014
Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Co-occurrence of plasmid-mediated colistin resistance gene, mcr-1 and blaScientific reports · 2026Article
- Allelic variations of intimin virulence gene (eae) in typical and atypical enteropathogenic Escherichia coli isolated from children with and without diarrhea in a rural area of southern Mozambique.BMC infectious diseases · 2026Article
- An Optimized PCR Assay to DetectFood safety (Tokyo, Japan) · 2025Article
- In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes ofMicroorganisms · 2025Article
- Prevalence, sequence diversity, and amplification of an IS-associated enterotoxin gene,Frontiers in microbiology · 2025Article
- Genomic Analysis of a Highly Virulent NDM-1-ProducingFrontiers in microbiology · 2022Article
- EAST1 toxin: An enigmatic molecule associated with sporadic episodes of diarrhea in humans and animals.Journal of microbiology (Seoul, Korea) · 2019Review
- Analysis of virulence potential of Escherichia coli O145 isolated from cattle feces and hide samples based on whole genome sequencing.PloS one · 2019Article
- Genetic Analysis of Virulence Potential ofFrontiers in microbiology · 2018Article
- High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil.BMC infectious diseases · 2017Article
- Diarrheagenic Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2016Review
- Evolution of diarrheagenicJournal of laboratory physiciansArticle
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) encoded by astA gene has been found in enteropathogenic E. coli (EPEC) strains. However, it is not sufficient to simply probe strains with an astA gene probe due to the existence of astA mutants (type 1 and type 2 SHEAST) and EAST1 variants (EAST1 v1-4). In this study, 222 EPEC (70 typical and 152 atypical) isolates were tested for the presence of the astA gene sequence by PCR and sequencing.
resultsThe astA gene was amplified from 54 strains, 11 typical and 43 atypical. Sequence analysis of the PCR products showed that 25 strains, 7 typical and 18 atypical, had an intact astA gene. A subgroup of 7 atypical strains had a variant type of the astA gene sequence, with four non-synonymous nucleotide substitutions. The remaining 22 strains had mutated astA gene with nucleotide deletions or substitutions in the first 8 codons. The RT-PCR results showed that the astA gene was transcribed only by the strains carrying either the intact or the variant type of the astA gene sequence. Southern blot analysis indicated that astA is located in EAF plasmid in typical strains, and in plasmids of similar size in atypical strains. Strains carrying intact astA genes were more frequently found in diarrheic children than in non-diarrheic children (p < 0.05).
conclusionIn conclusion, our data suggest that the presence of an intact astA gene may represent an additional virulence determinant in both EPEC groups.
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