ArticleMalaria journal2014
Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys.
Article in Malaria journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 2 of them syntheses that pooled it.
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Who cites it
42 citing papers in PubMed, 2 syntheses or guidelines pooled it, 69 citations in OpenAlex.
- Prevalence and proportion estimate of asymptomatic Plasmodium infection in Asia: a systematic review and meta-analysis.Scientific reports · 2023Pooled it
- A systematic review of sub-microscopic Plasmodium vivax infection.Malaria journal · 2015Pooled it
- UndetectedMalariaWorld journal · 2025Article
- Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review.Tropical medicine and infectious disease · 2024Review
- Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots.The American journal of tropical medicine and hygiene · 2024Article
- Diagnostic performance of ultrasensitive rapid diagnostic test for the detection of Plasmodium falciparum infections in asymptomatic individuals in Kisangani, Northeast Democratic Republic of Congo.Malaria journal · 2023Article
- An active and targeted survey reveals asymptomatic malaria infections among high-risk populations in Mondulkiri, Cambodia.Malaria journal · 2023Article
- The presence of Plasmodium malariae and Plasmodium knowlesi in near malaria elimination setting in western Indonesia.Malaria journal · 2022Article
- The Impact of Submicroscopic Parasitemia on Malaria Rapid Diagnosis in Northeastern Tanzania, an Area with Diverse Transmission Patterns.Infectious disease reports · 2022Article
- A systematic review and meta-analysis on sub-microscopicThe Lancet regional health. Southeast Asia · 2022Article
- Dry season prevalence of Plasmodium falciparum in asymptomatic gambian children, with a comparative evaluation of diagnostic methods.Malaria journal · 2022Article
- Nationwide molecular surveillance of three Plasmodium species harboured by symptomatic malaria patients living in Ghana.Parasites & vectors · 2022Article
- Advances and challenges in automated malaria diagnosis using digital microscopy imaging with artificial intelligence tools: A review.Frontiers in microbiology · 2022Review
- 'Bouncing Back' From Subclinical Malaria: Inflammation and Erythrocytosis After Resolution ofFrontiers in immunology · 2022Article
- Molecular Surveillance andFrontiers in cellular and infection microbiology · 2021Article
- Multiplexed quantitative proteomics provides mechanistic cues for malaria severity and complexity.Communications biology · 2020Article
- Prevalence of Asymptomatic and/or Low-Density Malaria Infection among High-Risk Groups in Peninsular Malaysia.The American journal of tropical medicine and hygiene · 2020Article
- Characteristics of imported Plasmodium ovale spp. and Plasmodium malariae in Hubei Province, China, 2014-2018.Malaria journal · 2020Article
- Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiOScientific reports · 2020Article
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Authors and funding
14 authors at 7 institutions in 4 countries.
Funding
Abstract
backgroundNested PCR is considered a sensitive and specific method for detecting malaria parasites and is especially useful in epidemiological surveys. However, the preparation of DNA templates for PCR is often time-consuming and costly.
methodsA simplified PCR method was developed to directly use a small blood filter paper square (2 × 2 mm) as the DNA template after treatment with saponin. This filter paper-based nested PCR method (FP-PCR) was compared to microscopy and standard nested PCR with DNA extracted by using a Qiagen DNA mini kit from filter paper blood spots of 204 febrile cases. The FP-PCR technique was further applied to evaluate malaria infections in 1,708 participants from cross-sectional epidemiological surveys conducted in Myanmar and Thailand.
resultsThe FP-PCR method had a detection limit of ~0.2 parasites/μL blood, estimated using cultured Plasmodium falciparum parasites. With 204 field samples, the sensitivity of the FP-PCR method was comparable to that of the standard nested PCR method, which was significantly higher than that of microscopy. Application of the FP-PCR method in large cross-sectional studies conducted in Myanmar and Thailand detected 1.9% (12/638) and 6.2% (66/1,070) asymptomatic Plasmodium infections, respectively, as compared to the detection rates of 1.3% (8/638) and 0.04% (4/1,070) by microscopy.
conclusionThis FP-PCR method was much more sensitive than microscopy in detecting Plasmodium infections. It drastically increased the detection sensitivity of asymptomatic infections in cross-sectional surveys conducted in Thailand and Myanmar, suggesting that this FP-PCR method has a potential for future applications in malaria epidemiology studies.
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