ArticleAnnals of clinical microbiology and antimicrobials2014
Molecular characterization of clinical multidrug-resistant Klebsiella pneumoniae isolates.
Article in Annals of clinical microbiology and antimicrobials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals.Scientific reports · 2016Trial
- Genomic Characterization of Pan-Drug Resistant Klebsiella pneumoniae KPNW Isolated From UTI Patient in Bangladesh.MicrobiologyOpen · 2025Article
- Whole Genome Sequencing ofAntibiotics (Basel, Switzerland) · 2025Article
- Molecular diagnostics improve the yield of diagnosis of community-acquired pneumonia and multidrug-resistant pathogens in hospitalised patients with HIV in a low-income setting.African journal of thoracic and critical care medicine · 2025Article
- The Antibacterial Activity ofMolecules (Basel, Switzerland) · 2023Article
- Antimicrobial Resistance Pattern, Clustering Mechanisms and Correlation Matrix of Drug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Healthcare Facilities as Potential Reservoirs of Antimicrobial ResistantPharmaceuticals (Basel, Switzerland) · 2022Article
- High Prevalence ofFoods (Basel, Switzerland) · 2022Article
- Clinical carbapenem-resistantFrontiers in cellular and infection microbiology · 2022Article
- Characterization and Full Genome Sequence of Novel KPP-5 Lytic Phage againstBiomedicines · 2021Article
- Genotypic Characterization of ClinicalFrontiers in microbiology · 2021Article
- Observational
- Prenatal and postnatal determinants in shaping offspring's microbiome in the first 1000 days: study protocol and preliminary results at one month of life.Italian journal of pediatrics · 2020Observational
- Characterization of KPC-ProducingFrontiers in microbiology · 2020Article
- Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-ResistantBioMed research international · 2020Article
- Gene Composition as a Potential Barrier to Large Recombinations in the Bacterial Pathogen Klebsiella pneumoniae.Genome biology and evolution · 2019Article
- Multiple Benefits of Plasmid-Mediated Quinolone Resistance Determinants inFrontiers in microbiology · 2019Article
- The nature and epidemiology of OqxAB, a multidrug efflux pump.Antimicrobial resistance and infection control · 2019Review
- Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistantAntimicrobial resistance and infection control · 2019Article
- Phenotypic and Genotypic Characterization ofFrontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKlebsiella pneumoniae is a frequent nosocomial pathogen, with the multidrug-resistant (MDR) K. pneumoniae being a major public health concern, frequently causing difficult-to-treat infections worldwide. The aim of this study was to investigate the molecular characterization of clinical MDR Klebsiella pneumoniae isolates.
methodsA total of 27 non-duplicate MDR K. pneumoniae isolates with a CTX-CIP-AK resistance pattern were investigated for the prevalence of antimicrobial resistance genes including extended spectrum β-lactamase genes (ESBLs), plasmid-mediated quinolone resistance (PMQR) genes, 16S rRNA methylase (16S-RMTase) genes, and integrons by polymerase chain reaction (PCR) amplification and DNA sequencing. Plasmid replicons were typed by PCR-based replicon typing (PBRT). Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to characterize the strain relatedness.
resultsAll the isolates co-harbored 3 or more resistance determinants. OqxAB, CTX-M-type ESBLs and RmtB were the most frequent determinants, distributed among 19 (70.4%),18 (66.7%) and 8 (29.6%) strains. Fourteen isolates harbored class 1 integrons, with orfD-aacA4 being the most frequent gene cassette array. Class 3 integrons were less frequently identified and contained the gene cassette array of blaGES-1-blaOXA-10-aac(6')-Ib. IncFII replicon was most commonly found in this collection. One cluster was observed with ≥80% similarity among profiles obtained by PFGE, and one sequence type (ST) by MLST, namely ST11, was observed in the cluster.
conclusionK. pneumoniae carbapenemase (KPC)-producing ST11 was the main clone detected. Of particular concern was the high prevalence of multiple resistance determinants, classs I integrons and IncFII plasmid replicon among these MDR strains, which provide advantages for the rapid development of MDR strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.