ArticleBMC cancer2014
The nucleolar size is associated to the methylation status of ribosomal DNA in breast carcinomas.
Article in BMC cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- The role of ribosomal DNA methylation in embryonic development, aging and diseases.Epigenetics & chromatin · 2024Review
- Quadrato Motor Training (QMT) is associated with DNA methylation changes at DNA repeats: A pilot study.PloS one · 2023Article
- Nucleolus assessment in invasive breast carcinoma, an objective parameter for histological grading.Turkish journal of medical sciences · 2022Article
- DNA Methylation Analysis of Ribosomal DNA in Adults With Down Syndrome.Frontiers in genetics · 2022Article
- Article
- Aging and Caloric Restriction Modulate the DNA Methylation Profile of the Ribosomal RNA Locus in Human and Rat Liver.Nutrients · 2020Article
- Ribosomal DNA harbors an evolutionarily conserved clock of biological aging.Genome research · 2019Article
- Differential hypomethylation of the repetitiveComparative cytogenetics · 2018Article
- Article
- Article
- Mammary epithelial morphogenesis and early breast cancer. Evidence of involvement of basal components of the RNA Polymerase I transcription machinery.Cell cycle (Georgetown, Tex.) · 2016Article
- The Epigenetic Pathways to Ribosomal DNA Silencing.Microbiology and molecular biology reviews : MMBR · 2016Review
- Association between the CpG island methylator phenotype and its prognostic significance in primary pulmonary adenocarcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Nucleolus-derived mediators in oncogenic stress response and activation of p53-dependent pathways.Histochemistry and cell biology · 2016Review
- Linker histone variant H1T targets rDNA repeats.Epigenetics · 2016Article
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- Aberrant DNA Methylation of rDNA and PRIMA1 in Borderline Personality Disorder.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is a body of evidence that shows a link between tumorigenesis and ribosome biogenesis. The precursor of mature 18S, 28S and 5.8S ribosomal RNAs is transcribed from the ribosomal DNA gene (rDNA), which exists as 300-400 copies in the human diploid genome. Approximately one half of these copies are epigenetically silenced, but the exact role of epigenetic regulation on ribosome biogenesis is not completely understood. In this study we analyzed the methylation profiles of the rDNA promoter and of the 5' regions of 18S and 28S in breast cancer.
methodsWe analyzed rDNA methylation in 68 breast cancer tissues of which the normal counterpart was partially available (45/68 samples) using the MassARRAY EpiTYPER assay, a sensitive and quantitative method with single base resolution.
resultsWe found that rDNA locus tended to be hypermethylated in tumor compared to matched normal breast tissues and that the DNA methylation level of several CpG units within the rDNA locus was associated to nuclear grade and to nucleolar size of tumor tissues. In addition we identified a subgroup of samples in which large nucleoli were associated with very limited or absent rDNA hypermethylation in tumor respect to matched normal tissue.
conclusionsIn conclusion, we suggest that rDNA is an important target of epigenetic regulation in breast tumors and that rDNA methylation level is associated to nucleolar size.
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