ArticleBMC genomics2014
High-throughput capturing and characterization of mutations in essential genes of Caenorhabditis elegans.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
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- Genome-Wide Protein Interaction Analysis in Parasitic Gyrodactylus Flatworms-Fish Hosts System and Drug Target Identification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A hyperpolarizing neuron recruits undocked innexin hemichannels to transmit neural information inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers.Methods in molecular biology (Clifton, N.J.) · 2022Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- High-dose folic acid supplementation results in significant accumulation of unmetabolized homocysteine, leading to severe oxidative stress in Caenorhabditis elegans.Redox biology · 2020Article
- Kettin, the large actin-binding protein with multiple immunoglobulin domains, is essential for sarcomeric actin assembly and larval development in Caenorhabditis elegans.The FEBS journal · 2020Article
- Genomic identification and functional analysis of essential genes in Caenorhabditis elegans.BMC genomics · 2018Article
- Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans.BMC biology · 2018Article
- Genomic Identification and Functional Characterization of Essential Genes inG3 (Bethesda, Md.) · 2018Article
- Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.Current protocols in molecular biology · 2017Article
- Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans.Genetics · 2016Review
- First insights into the nature and evolution of antisense transcription in nematodes.BMC evolutionary biology · 2016Article
- Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing.G3 (Bethesda, Md.) · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundEssential genes are critical for the development of all organisms and are associated with many human diseases. These genes have been a difficult category to study prior to the availability of balanced lethal strains. Despite the power of targeted mutagenesis, there are limitations in identifying mutations in essential genes. In this paper, we describe the identification of coding regions for essential genes mutated using forward genetic screens in Caenorhabditis elegans. The lethal mutations described here were isolated and maintained by a wild-type allele on a rescuing duplication.
resultsWe applied whole genome sequencing to identify the causative molecular lesion resulting in lethality in existing C. elegans mutant strains. These strains are balanced and can be easily maintained for subsequent characterization. Our method can be effectively used to analyze mutations in a large number of essential genes. We describe here the identification of 64 essential genes in a region of chromosome I covered by the duplication sDp2. Of these, 42 are nonsense mutations, six are splice signal mutations, one deletion, and 15 are non-synonymous mutations. Many of the essential genes in this region function in cell cycle, transcriptional regulation, and RNA processing.
conclusionsThe essential genes identified here are represented by mutant strains, many of which have more than one mutant allele. The genetic resource can be utilized to further our understanding of essential gene function and will be applicable to the study of C. elegans development, conserved cellular function, and ultimately lead to improved human health.
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