ArticleBMC microbiology2014
Multilocus sequence typing and ftsI sequencing: a powerful tool for surveillance of penicillin-binding protein 3-mediated beta-lactam resistance in nontypeable Haemophilus influenzae.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Revisiting mutational resistance to ampicillin and cefotaxime in Haemophilus influenzae.Genome medicine · 2024Pooled it
- Epidemiology of invasive Haemophilus influenzae infections in Belgium: 2018-2022.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Genomic profiling of cefotaxime-resistantFrontiers in microbiology · 2025Article
- Antibiotic resistance and molecular characterization of non-invasive clinicalJAC-antimicrobial resistance · 2024Article
- Multiple interspecies recombination events documented by whole-genome sequencing in multidrug-resistantAccess microbiology · 2024Article
- Molecular Epidemiology and Antibiotic Resistance Analysis of Non-TypeableAntibiotics (Basel, Switzerland) · 2023Article
- Can non-typeablePediatric investigation · 2023Article
- National Danish surveillance of invasive clinicalFrontiers in microbiology · 2023Article
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- Molecular epidemiology and antibiotic resistance profiles of invasiveFrontiers in microbiology · 2022Article
- Characterization ofLife (Basel, Switzerland) · 2021Article
- Haemophilus influenzae one day in Denmark: prevalence, circulating clones, and dismal resistance to aminopenicillins.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Polymorphism ofNew microbes and new infections · 2020Article
- Widespread of non-typeable Haemophilus influenzae with high genetic diversity after two decades use of Hib vaccine in China.Journal of clinical laboratory analysis · 2020Article
- Role of Horizontal Gene Transfer in the Development of Multidrug Resistance in Haemophilus influenzae.mSphere · 2020Article
- Multilocus Sequence Typing of Mycoplasma pneumoniae, Japan, 2002-2016.Emerging infectious diseases · 2018Article
- Benzylpenicillin versus wide-spectrum beta-lactam antibiotics as empirical treatment of Haemophilus influenzae-associated lower respiratory tract infections in adults; a retrospective propensity score-matched study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- The return of Pfeiffer's bacillus: Rising incidence of ampicillin resistance in Haemophilus influenzae.Microbial genomics · 2018Review
- Multiclonal Expansion and High Prevalence of β-Lactamase-Negative Haemophilus influenzae with High-Level Ampicillin Resistance in Japan and Susceptibility to Quinolones.Antimicrobial agents and chemotherapy · 2018Article
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9 authors.
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Abstract
backgroundBeta-lactam resistance in Haemophilus influenzae due to ftsI mutations causing altered penicillin-binding protein 3 (PBP3) is increasing worldwide. Low-level resistant isolates with the N526K substitution (group II low-rPBP3) predominate in most geographical regions, while high-level resistant isolates with the additional S385T substitution (group III high-rPBP3) are common in Japan and South Korea.Knowledge about the molecular epidemiology of rPBP3 strains is limited. We combined multilocus sequence typing (MLST) and ftsI/PBP3 typing to study the emergence and spread of rPBP3 in nontypeable H. influenzae (NTHi) in Norway.
resultsThe prevalence of rPBP3 in a population of 795 eye, ear and respiratory isolates (99% NTHi) from 2007 was 15%. The prevalence of clinical PBP3-mediated resistance to ampicillin was 9%, compared to 2.5% three years earlier. Group II low-rPBP3 predominated (96%), with significant proportions of isolates non-susceptible to cefotaxime (6%) and meropenem (20%). Group III high-rPBP3 was identified for the first time in Northern Europe.Four MLST sequence types (ST) with characteristic, highly diverging ftsI alleles accounted for 61% of the rPBP3 isolates. The most prevalent substitution pattern (PBP3 type A) was present in 41% of rPBP3 isolates, mainly carried by ST367 and ST14. Several unrelated STs possessed identical copies of the ftsI allele encoding PBP3 type A.Infection sites, age groups, hospitalization rates and rPBP3 frequencies differed between STs and phylogenetic groups.
conclusionsThis study is the first to link ftsI alleles to STs in H. influenzae. The results indicate that horizontal gene transfer contributes to the emergence of rPBP3 by phylogeny restricted transformation.Clonally related virulent rPBP3 strains are widely disseminated and high-level resistant isolates emerge in new geographical regions, threatening current empiric antibiotic treatment. The need of continuous monitoring of beta-lactam susceptibility and a global system for molecular surveillance of rPBP3 strains is underlined. Combining MLST and ftsI/PBP3 typing is a powerful tool for this purpose.
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