ArticleJournal of virological methods2014
Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections.
Article in Journal of virological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Prevalence, etiology, and transmission of fibropapillomatosis in Olive Ridley turtles at a mass-nesting colony in the Mexican Pacific.PloS one · 2026Article
- Fibropapillomatosis: A Review of the Disease with Attention to the Situation Northern Coast of Brazil.Animals : an open access journal from MDPI · 2024Review
- Case report: Diagnosis and autogenous vaccine treatment of herpesvirus in a green turtle (Frontiers in veterinary science · 2024Article
- Increased Prevalence and New Evidence of Multi-Species Chelonid Herpesvirus 5 (ChHV5) Infection in the Sea Turtles of Mabul Island, Borneo.Animals : an open access journal from MDPI · 2023Article
- Threats and Vulnerabilities for the Globally Distributed Olive Ridley (Animals : an open access journal from MDPI · 2022Review
- Preparation of recombinant glycoprotein B (gB) of Chelonid herpesvirus 5 (ChHV5) for antibody production and its application for infection detection in sea turtles.Scientific reports · 2022Article
- First Evidence of Chelonid Herpesvirus 5 (ChHV5) Infection in Green Turtles (Pathogens (Basel, Switzerland) · 2021Article
- Fibropapillomatosis and the Chelonid Alphaherpesvirus 5 in Green Turtles from West Africa.EcoHealth · 2021Article
- The Concurrent Detection of Chelonid Alphaherpesvirus 5 andAnimals : an open access journal from MDPI · 2021Article
- Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Animals : an open access journal from MDPI · 2021Article
- Herpesviruses in Captive Chelonians in Europe Between 2016 and 2020.Frontiers in veterinary science · 2021Article
- Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula.EcoHealth · 2020Article
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- Perspectives on the expansion of human precision oncology and genomic approaches to sea turtle fibropapillomatosis.Communications biology · 2019Article
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- Global distribution of Chelonid fibropapilloma-associated herpesvirus among clinically healthy sea turtles.BMC evolutionary biology · 2014Article
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Corrections and comments
- Erratum issued
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Chelonid fibropapilloma-associated herpesvirus (CFPHV) is hypothesized to be the causative agent of fibropapillomatosis, a neoplastic disease in sea turtles, given its consistent detection by PCR in fibropapilloma tumours. CFPHV has also been detected recently by PCR in tissue samples from clinically healthy (non exhibiting fibropapilloma tumours) turtles, thus representing presumably latent infections of the pathogen. Given that template copy numbers of viruses in latent infections can be very low, extremely sensitive PCR assays are needed to optimize detection efficiency. In this study, efficiency of several PCR assays designed for CFPHV detection is explored and compared to a method published previously. The results show that adoption of a triplet set of singleplex PCR assays outperforms other methods, with an approximately 3-fold increase in detection success in comparison to the standard assay. Thus, a new assay for the detection of CFPHV DNA markers is presented, and adoption of its methodology is recommended in future CFPHV screens among sea turtles.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.