ArticleAnalytical chemistry2014
Highly sensitive immunoassay based on controlled rehydration of patterned reagents in a 2-dimensional paper network.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 95 citations in OpenAlex.
- Paper-based microfluidics for wearable soft bioelectronics.Lab on a chip · 2026Review
- Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Capillary flow-driven immunoassay platform for COVID-19 antigen diagnostics.Analytica chimica acta · 2023Article
- Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing.Analytical methods : advancing methods and applications · 2023Article
- A low-cost, paper-based hybrid capture assay to detect high-risk HPV DNA for cervical cancer screening in low-resource settings.Lab on a chip · 2023Article
- Paper based microfluidic devices: a review of fabrication techniques and applications.The European physical journal. Special topics · 2023Review
- Multi-Omics Advancements towardsDiagnostics (Basel, Switzerland) · 2021Review
- Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids.Scientific reports · 2021Article
- Recent Advances of Fluid Manipulation Technologies in Microfluidic Paper-Based Analytical Devices (μPADs) toward Multi-Step Assays.Micromachines · 2020Review
- Characterization methods in porous materials for the rational design of multi-step processing in the context of a paper microfluidic phenylalanine test.Analytical methods : advancing methods and applications · 2020Article
- The Rapid Coronavirus Antibody Test: Can We Improve Accuracy?Frontiers in medicine · 2020Article
- Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments.Mikrochimica acta · 2019Review
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- From Point-of-Care Testing to eHealth Diagnostic Devices (eDiagnostics).ACS central science · 2018Review
- Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia.Sensors (Basel, Switzerland) · 2018Review
- Rapid flow in multilayer microfluidic paper-based analytical devices.Lab on a chip · 2018Article
- Enabling Microfluidics: from Clean Rooms to Makerspaces.Trends in biotechnology · 2017Review
- Highly Sensitive Two-Dimensional Paper Network Incorporating Biotin-Streptavidin for the Detection of Malaria.Analytical chemistry · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have demonstrated a multistep 2-dimensional paper network immunoassay based on controlled rehydration of patterned, dried reagents. Previous work has shown that signal enhancement improves the limit of detection in 2-dimensional paper network assays, but until now, reagents have only been included as wet or dried in separate conjugate pads placed at the upstream end of the assay device. Wet reagents are not ideal for point-of-care because they must be refrigerated and typically limit automation and require more user steps. Conjugate pads allow drying but do not offer any control of the reagent distribution upon rehydration and can be a source of error when pads do not contact the assay membrane uniformly. Furthermore, each reagent is dried on a separate pad, increasing the fabrication complexity when implementing multistep assays that require several different reagents. Conversely, our novel method allows for consistent, controlled rehydration from patterned reagent storage depots directly within the paper membrane. In this assay demonstration, four separate reagents were patterned in different regions of the assay device: a gold-antibody conjugate used for antigen detection and three different signal enhancement components that must not be mixed until immediately before use. To show the viability of patterning and drying reagents directly onto a paper device for dry reagent storage and subsequent controlled release, we tested this device with the malaria antigen Plasmodium falciparum histidine-rich protein 2 (PfHRP2) as an example of target analyte. In this demonstration, the signal enhancement step increases the visible signal by roughly 3-fold and decreases the analytical limit of detection by 2.75-fold.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.