ArticleTissue engineering. Part A2014
Alginate encapsulation of human embryonic stem cells to enhance directed differentiation to pancreatic islet-like cells.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Airflow controlled droplet bioprinting enables high-throughput generation of iPSC-derived thymic organoids withBiofabrication · 2026Article
- Xeno-Free Peptide-Functionalized Hydrogels Support hiPSC Encapsulation andbioRxiv : the preprint server for biology · 2026Article
- Hydrostatic Pressure Induces Osteogenic Differentiation of Single Stem Cells in 3D Viscoelastic Microgels.Small science · 2025Article
- Supragel-mediated efficient generation of pancreatic progenitor clusters and functional glucose-responsive islet-like clusters.Bioactive materials · 2024Article
- Algal Phycocolloids: Bioactivities and Pharmaceutical Applications.Marine drugs · 2023Review
- Comparative Study of Two Common In Vitro Models for the Pancreatic Islet with MIN6.Tissue engineering and regenerative medicine · 2023Article
- Central tolerance promoted by cell chimerism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids.Nature methods · 2022Article
- Differentiation of the mesenchymal stem cells to pancreatic β-like cells in alginate/trimethyl chitosan/alginate microcapsules.Progress in biomaterials · 2022Article
- In vitro generation of transplantable insulin-producing cells from canine adipose-derived mesenchymal stem cells.Scientific reports · 2022Article
- Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.ACS biomaterials science & engineering · 2021Review
- Effect of alginate matrix engineered to mimic the pancreatic microenvironment on encapsulated islet function.Biotechnology and bioengineering · 2021Article
- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems.Bioengineering (Basel, Switzerland) · 2020Review
- Engineering Strategies to Improve Islet Transplantation for Type 1 Diabetes Therapy.ACS biomaterials science & engineering · 2020Article
- Microfluidics-based fabrication of cell-laden microgels.Biomicrofluidics · 2020Review
- Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells.Journal of biological engineering · 2020Article
- A Fiber Alginate Co-culture Platform for the Differentiation of mESC and Modeling of the Neural Tube.Frontiers in neuroscience · 2020Article
- Current Status of Alginate in Drug Delivery.Advances in pharmacological and pharmaceutical sciences · 2020Review
- Nano-fibre Integrated Microcapsules: A Nano-in-Micro Platform for 3D Cell Culture.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The pluripotent property of human embryonic stem cells (hESCs) makes them attractive for treatment of degenerative diseases such as diabetes. We have developed a stage-wise directed differentiation protocol to produce alginate-encapsulated islet-like cells derived from hESCs, which can be directly implanted for diabetes therapy. The advantage of alginate encapsulation lies in its capability to immunoisolate, along with the added possibility of scalable culture. We have evaluated the possibility of encapsulating hESCs at different stages of differentiation. Encapsulation of predifferentiated cells resulted in insufficient cellular yield and differentiation. On the other hand, encapsulation of undifferentiated hESCs followed by differentiation induction upon encapsulation resulted in the highest viability and differentiation. More striking was that alginate encapsulation resulted in a much stronger differentiation compared to parallel two-dimensional cultures, resulting in 20-fold increase in c-peptide protein synthesis. To elucidate the mechanism contributing to encapsulation-mediated enhancement in hESC maturation, investigation of the signaling pathways revealed interesting insight. While the phospho-protein levels of all the tested signaling molecules were lower under encapsulation, the ratio of pSMAD/pAKT was significantly higher, indicating a more efficient signal transduction under encapsulation. These results clearly demonstrate that alginate encapsulation of hESCs and differentiation to islet-cell types provides a potentially translatable treatment option for type 1 diabetes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.