ArticleFungal genetics and biology : FG & B2014
The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation.
Article in Fungal genetics and biology : FG & B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- TrLPMO9A drives oxidative cellulose depolymerization in Trichoderma reesei and is enhanced by heterologous cellobiose dehydrogenase expression.Biotechnology for biofuels and bioproducts · 2026Article
- Heterotrimeric G-proteins and cAMP regulate gene expression during growth on cellulose inmBio · 2026Article
- Correlating sugar transporter expression and activities to identify transporters for an orphan sugar substrate.Applied microbiology and biotechnology · 2024Article
- The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa.BMC biotechnology · 2023Article
- MAPkinases regulate secondary metabolism, sexual development and light dependent cellulase regulation in Trichoderma reesei.Scientific reports · 2023Article
- Post-Translational Modifications of Histones Are Versatile Regulators of Fungal Development and Secondary Metabolism.Toxins · 2022Review
- Proteomic Profiling of the Substantia Nigra to Identify Determinants of Lewy Body Pathology and Dopaminergic Neuronal Loss.Journal of proteome research · 2021Article
- RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.Biotechnology for biofuels · 2021Article
- A mitogen-activated protein kinase PoxMK1 mediates regulation of the production of plant-biomass-degrading enzymes, vegetative growth, and pigment biosynthesis in Penicillium oxalicum.Applied microbiology and biotechnology · 2021Article
- Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa.Applied and environmental microbiology · 2020Article
- The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa.PLoS genetics · 2019Article
- Article
- Communicate and Fuse: How Filamentous Fungi Establish and Maintain an Interconnected Mycelial Network.Frontiers in microbiology · 2019Review
- Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition inFrontiers in microbiology · 2019Article
- Regulation of Cell-to-Cell Communication and Cell Wall Integrity by a Network of MAP Kinase Pathways and Transcription Factors inGenetics · 2018Article
- mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability.PloS one · 2018Article
- Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism.PLoS genetics · 2017Article
- Identification and characterization of the glucose dual-affinity transport system inBiotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responses to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.