ArticleMolecular biotechnology2014
Miscanthus: genetic diversity and genotype identification using ISSR and RAPD markers.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.
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5 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Traditional System Versus DNA Barcoding in Identification of Bamboo Species: A Systematic Review.Molecular biotechnology · 2021Pooled it
- Impact of salt strength on in vitro propagation and rebaudioside A content in Stevia rebaudiana under semi-solid and liquid MS media.Scientific reports · 2024Article
- Article
- Genetic diversity ofPeerJ · 2016Article
- Characterization of 12 Novel Microsatellite Markers of Sogatella furcifera (Hemiptera: Delphacidae) Identified From Next-Generation Sequence Data.Journal of insect science (Online) · 2015Article
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3 authors.
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Abstract
Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus "Floridulus," which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus "Floridulus," which proved to be closely related to M. × giganteus. Similar groupings were evident in the PCoA analysis.
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