ArticleThe Journal of membrane biology2014
Effect of pulsed electric field treatments on permeabilization and extraction of pigments from Chlorella vulgaris.
Article in The Journal of membrane biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 151 citations in OpenAlex.
- Electrostimulation waveform optimization for enhancing biomass and macromolecule production in Chlorella vulgaris.Bioprocess and biosystems engineering · 2026Article
- Green Optimization of Sesame Seed Oil Extraction via Pulsed Electric Field and Ultrasound Bath: Yield, Antioxidant Activity, Oxidative Stability, and Functional Food Potential.Foods (Basel, Switzerland) · 2025Article
- Pulsed Electric Field-Assisted "Green" Extraction of Betalains and Phenolic Compounds fromFoods (Basel, Switzerland) · 2025Article
- High-yield zeaxanthin production in Chlamydomonas reinhardtii via advanced metabolic pathway engineering.Biotechnology for biofuels and bioproducts · 2025Article
- Biphasic lipid extraction from microalgae after PEF-treatment reduces the energy demand of the downstream process.Biotechnology for biofuels and bioproducts · 2025Article
- Pulsed electric field (PEF) processing of microalga Chlorella vulgaris and its digestibility in broiler feed.Poultry science · 2024Article
- Sustainable Green Extraction of Carotenoid Pigments: Innovative Technologies and Bio-Based Solvents.Antioxidants (Basel, Switzerland) · 2024Review
- Microbial Pigments: Major Groups and Industrial Applications.Microorganisms · 2023Review
- Sustainable Production of Pigments from Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Novel Approaches for the Recovery of Natural Pigments with Potential Health Effects.Journal of agricultural and food chemistry · 2022Review
- Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene fromMarine drugs · 2021Article
- Assessment of Phycocyanin Extraction fromMicroorganisms · 2021Article
- Carotenoids from Cyanobacteria: Biotechnological Potential and Optimization Strategies.Biomolecules · 2021Review
- Pulsed Electric Field-Assisted Extraction of Aroma and Bioactive Compounds From Aromatic Plants and Food By-Products.Frontiers in nutrition · 2021Article
- The generation of metabolic changes for the production of high-purity zeaxanthin mediated by CRISPR-Cas9 in Chlamydomonas reinhardtii.Microbial cell factories · 2020Article
- Improved Extraction Efficiency of Antioxidant Bioactive Compounds fromMolecules (Basel, Switzerland) · 2020Article
- Real-Time Impedance Monitoring During Electroporation Processes in Vegetal Tissue Using a High-Performance Generator.Sensors (Basel, Switzerland) · 2020Article
- Enhancement of the Antioxidant, Anti-Tyrosinase, and Anti-Hyaluronidase Activity ofMolecules (Basel, Switzerland) · 2020Article
- Enzymatic Processes Triggered by PEF for Astaxanthin Extraction FromFrontiers in bioengineering and biotechnology · 2020Article
- Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants.Frontiers in bioengineering and biotechnology · 2020Article
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4 authors at 1 institution in 1 country.
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Abstract
The effect of pulsed electric field (PEF) treatments of different intensities on the electroporation of the cytoplasmatic membrane of Chlorella vulgaris, and on the extraction of carotenoids and chlorophylls were investigated. Staining the cells with propidium iodide before and after the PEF treatment revealed the existence of reversible and irreversible electroporation. Application of PEF treatments in the range of 20-25 kV cm(-1) caused most of the population of C. vulgaris to be irreversibly electroporated even at short treatment times (5 pulses of 3 µs). However, at lower electric field strengths (10 kV cm(-1)), cells that were reversibly electroporated were observed even after 50 pulses of 3 µs. The electroporation of C. vulgaris cells by PEF higher than 15 kV cm(-1) and duration is higher than 15 µs increased significantly the extraction yield of intracellular components of C. vulgaris. The application of a 20 kV cm(-1) for 75 μs increased the extraction yield just after the PEF treatment of the carotenoids, and chlorophylls a and b 0.5, 0.7, and 0.8 times, respectively. However, further increments in electric field strength and treatment time did not cause significant increments in the extraction yield. The extraction of carotenoids from PEF-treated C. vulgaris cells after 1 h of the application of the treatment significantly increased the extraction yield in comparison to the yield obtained from the cells extracted just after the PEF treatment. After PEF treatment at 20 kV cm(-1) for 75 µs, extraction yield for carotenoids, and chlorophylls a and b increased 1.2, 1.6, and 2.1 times, respectively. A high correlation was observed between irreversible electroporation and percentage of yield increase when the extraction was conducted after 1 h of the application of PEF treatment (R: 0.93), but not when the extraction was conducted just after PEF treatment (R: 0.67).
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